Chitosan/hydroxyapatite composite scaffolds for articular cartilage injury

doi:10.3969/j.issn.2095-4344.2017.02.015

Abstract

Abstract:

BACKGROUND: Studies have shown that the chitosan/hydroxyapatite composite scaffold with good biological performance is very similar to natural bone in structure, and plays the performance of different materials.
OBJECTIVE: To observe the repairing effect of chitosan/hydroxyapatite composite scaffold on articular cartilage injury.
METHODS: Thirty New Zealand white rabbits were selected to make articular cartilage injury on the left knee, and then model rabbits were randomly divided into sodium hyaluronate gel group (control group, n=15) and chitosan/hydroxyapatite composite scaffold group (experimental group, n=15). At 4, 8, 12 weeks after scaffold implantation, cartilage tissues were taken out and observed histologically.
RESULTS AND CONCLUSION: Compared with the control group, the experimental group showed higher Wakitani scores at 4, 8, 12 weeks, but lower Mankin scores at 4 and 12 weeks after implantation (both P < 0.05). Safranin-O staining results showed that at 4 weeks after implantation, chondrocytes arranged relatively in disorder with overt pleated small projections in the control group, while chondrocytes were well aligned with slightly pleated small projections and slightly large nuclei in the experimental group; at 8 weeks, chondrocytes arranged irregularly with pleated small projections in the control group, while in the experimental group, the chondrocytes arranged orderly with pleated small projections and large nuclei; at 12 weeks, the chondrocytes in the control group exhibited a substantially ordered arrangement with pleated small projections and large nuclei, while in the experimental group, the chondrocytes arranged in order with no pleated small projections but with large nuclei. All these findings indicate that chitosan/hydroxyapatite composite scaffold can promote articular cartilage repair.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Hydroxyapatites, Chitosan, Tissue Engineering

CLC Number:

R318

Liu Hong-ya. Chitosan/hydroxyapatite composite scaffolds for articular cartilage injury[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(2): 244-248.

Figures/Tables 3

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