Vitreous cryopreservation of tenocytes co-cultured with porous polydimethylsiloxane scaffolds

doi:10.3969/j.issn.2095-4344.2017.02.014

Abstract

Abstract:

BACKGROUND: Accumulative evidence supports that vitreous cryopreservation can improve the cell survival rate.
OBJECTIVE: To investigate the effect of vitreous cryopreservation on the tenocytes co-cultured with the porous polydimethylsiloxane (PDMS) scaffold.
METHODS: Tenocytes were co-cultured with the porous PDMS scaffold for 9-14 days, and then preserved and resuscitated in the 10% dimethyl sulfoxide (DMSO), 21% DMSO and VS55, respectively. One hour later, the survival rate of post-resuscitated tenocytes versus pre-resusciated tenocytes was analyzed by live/dead double color fluorescent staining and flow cytometry.
RESULTS AND CONCLUSION: Live/dead double color fluorescent staining revealed that tenocytes in the 10% DMSO group appeared to be irregular and double stained, and a large number of cells shedding from the scaffold. The VS55 and 21% DMSO groups showed some spindle and hemispherical cells single stained for green fluorescence and few double stained irregular cells. Additionally, the cell density in the two groups was significantly lower than that in the control group. Flow cytometry results found that there were homogenous cells in the control group; the number of cells in the 10% DMSO group was too low to undergo flow cytometry; small cell particles were visible in the VS55 group; in the 21% DMSO group, the cell volume was similar with the control group, and small particles also existed. The survival rate in the VS55 group (64.9%) was significantly lower than that in the 21% DMSO group (76.2%; P < 0.05). Conversely, the survived cells were rare in the 10% DMSO group. To conclude, 21% DMSO vitreous cryopreservation improves the cell survival rate and is beneficial for tenocyte adherence to the scaffold.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Cryopreservation, Dimethyl Sulfoxide, Tissue Engineering

CLC Number:

R318

Wang Zhi, Tan Mei-yun, Qing Quan, Chen Xi, Liu Cheng-jun, Qin Ting-wu. Vitreous cryopreservation of tenocytes co-cultured with porous polydimethylsiloxane scaffolds[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(2): 238-243.

Figures/Tables 7

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