In vivo tumorigenicity of bone marrow mesenchymal stem cells in the body after the conversion of gastric cancer microenvironment

doi:10.3969/j.issn.2095-4344.2017.01.006

Abstract

Abstract:

BACKGROUND: The clinical gastric cancer tissues and the mesenchymal stem cells isolated from tumor
tissues in the body of nude mice have similar characteristics with bone marrow mesenchymal stem cells, which are certificated to be an important part of the tumor microenvironment that can promote the growth of tumor.
OBJECTIVE: To observe that whether bone marrow mesenchymal stem cells have the tumorigenic ability after the conversion of gastric microenvironment.
METHODS: Bone marrow mesenchymal stem cells cultured singly and routinely were used as controls. Rat bone marrow mesenchymal stem cells were indirectly co-cultured with gastric cancer BCG-823 cells in a Transwell chamber to construct the gastric microenvironment (experimental group). Bone marrow mesenchymal stem cells from each group were subcutaneously seeded into the forelimb armpit of nude mice, and then, the formation of subcutaneous nodules was observed. Immunohistochemical method was used to detect the expression of tumor tissue latent membrane protein 1 (LMP1) and tumor suppressor gene TCF21. Transmission electron microscope was used to observe the ultrastructure of tumor tissues.
RESULTS AND CONCLUSION: After 7-14 days, there were no tumor nodules in the nude mouse armpit in the control group. On the contrary, in the experimental group, tumor nodules formed in the nude mouse armpit after 7 days, and these nodules were enlarged and kept stable until the 14th day. The immunohistochemical results showed high level of LMP1, but low level of TCF21. Under the transmission electron microscope, the tumor tissues had a large volume and an irregular shape, the cell mricovilli were increased abnormally, and there were many fendritic protrusions on the surface, indicating the presence of obvious intracellular changes. In conclusion, bone marrow mesenchymal stem cells after the conversion in the gastric microenvironment have the ability of tumorigenicity in vivo.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Stomach Neoplasms, Tissue Engineering

CLC Number:

R394.2

Wang Yong-feng, Liu Xi-ping, Cui Guo-ning, Dong Jun-gang, Li Pei-qing, Ming Hai-xia, Zhang Wei . In vivo tumorigenicity of bone marrow mesenchymal stem cells in the body after the conversion of gastric cancer microenvironment[J]. Chinese Journal of Tissue Engineering Research, 2017, 21(1): 32-37.

Figures/Tables 1

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