Isolation, identification and culture of porcine heart valve myofibroblasts

doi:10.3969/j.issn.2095-4344.2016.51.013

Abstract

Abstract:

BACKGROUND: Valvular interstitial cells are the main components of the heart valves. Myofibroblasts, as a kind of valvular interstitial cells, can express alpha-smooth muscle actin and type I collagen fiber, and hold differentiation potential. These cells cannot only play a support role in the valve structure, but also play a regulatory role in the process of the valve normal physiological and pathological responses.
OBJECTIVE: To obtain a reliable method of separation, primary culture and identification of myofibroblasts laying a foundation for further study on the cardiac valvular calcification.
METHODS: Aortic valve myofibroblas extracted from porcine hearts were primary cultured by trypsin and collagenase combined digestive method, common enzyme-digestion method and tissue-culture method, respectively. The myofibroblast activity and morphology were observed using microscope, and myofibroblasts were identified using light microscope and immunocytochemistrial method.
RESULTS AND CONCLUSION: Myofibroblasts had a higher activity and purity cultured by trypsin combined with collagenase II digestion method. Aortic valve myofibroblasts were positive for alpha-smooth muscle actin and negative for von Willebrand factor under fluorescence microscope, suggesting that myofibroblasts were successfully obtained.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Cell Culture Techniques, Cell Separation, Myocytes, Smooth Muscle, Tissue Engineering

CLC Number:

R318

Liu Feng-dan, Hu Wei-lin, Chen Zheng-ping, Li Yong-sheng. Isolation, identification and culture of porcine heart valve myofibroblasts[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(51): 7684-7689.

Figures/Tables 3

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