Expression of growth hormone and insulin-like growth factor-1 in the temporal cortex of a Lewis dwarf model rat

doi:10.3969/j.issn.2095-4344.2016.51.014

Abstract

Abstract:

BACKGROUND: Insulin-like growth factor-1(IGF-1), a main active factor in growth hormone (GH), plays various biological functions, such as improving cognitive ability and anti-apoptotic action.
OBJECTIVE: To detect the expressions of GH and IGF-1 in the temporal cortex of Lewis dwarf rats, and to explore the effect of different concentrations of GH on the differentiation of hippocampal nerve stem cells (NSCs).
METHODS: Lewis dwarf rats aged 11(adult) and 20 (senile) month olds and normal wild-type rats were euthanized by decapitation, underwent the craniotomy quickly, and the temporal cortex in the cold saline was extracted. GH and IGF-1 levels were detected using western blotting. After isolation, purification and identification of the rat hippocampal NSCs, the effect of GH in different concentrations (10, 30, 90 μg/L) on the NSCs differentiation was determined at 96 hours after culture.
RESULTS AND CONCLUSION: The GH level in the temporal cortex did not differ significantly among rats (P > 0.05). While the IGF-I level in the temporal cortex of Lewis dwarf rats was significantly higher than that of the wild-type rats (P < 0.05). The GH level in the temporal cortex of adult female Lewis dwarf rats was significantly lower than that of the male rats (P < 0.05). Immunofluorescence showed that the proportion of β III-tubulin-positive neurons was significantly higher than that in the control group (P < 0.05) after the hippocampal NSCs and precursor cells cultured for 96 hours with GH (30 μg/L), but there was no significant difference between the control group and treatment group with GH of 10 or 90 μg/L. These results suggest that GH and IGF-I are expressed in the temporal cortex of both Lewis dwarf and wild-type rats which are independent from pituitary GH and the peripheral circulating IGF-1. Additionally, GH can promote the differentiation of hippocampal NSCs and precursor cells into neurons.

中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Growth Hormone, Insulin-Like Growth Factor-1, Neural Stem Cells, Tissue Engineering

CLC Number:

R318

Cai Yi-qi, Wang Kai-fei, Wang Ying-ying, Zhang Su-hua . Expression of growth hormone and insulin-like growth factor-1 in the temporal cortex of a Lewis dwarf model rat[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(51): 7690-7696.

Figures/Tables 4

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