Tissue-engineered periosteum-coated biphasic calcium phosphate scaffold repairs bone defects in rabbits

doi:10.3969/j.issn.2095-4344.2016.47.002

Abstract

Abstract:

BACKGROUND: Periosteum has been shown to play an important role in bone defect repair, but large bone defects are often accompanied by the loss of periosteum. Therefore, tissue-engineered periosteum constructed by tissue engineering technology is attracting more attention.
OBJECTIVE: To investigate the effect of the tissue-engineered periosteum which is constructed by mesenchymal stem cells from the peripheral blood and procine small intestinal submucosa and biphasic calcium phosphates on the repair of bone defects in rabbits.
METHODS: Mesenchymal stem cells from peripheral blood were seeded onto the procine small intestinal submucosa to construct the tissue-engineered periosteum, and then the cell growth was observed through live/dead staining. The bone defect model of 15 mm in length in the left ulna was created in the 30 healthy New Zealand rabbits, followed by randomly assigned to 3 groups, and then treated with biphasic calcium phosphates and tissue-engineered periosteum-coated biphasic calcium phosphates, respectively. The remaining rabbits received no treatment as controls. Therapeutic effects were compared by X-ray and histological examinations among groups.
RESULTS AND CONCLUSION: Live/dead staining found that the mesenchymal stem cells grew well and the cell number increased gradually. X-ray Lane-Sandhu scores of the group treated with tissue-engineered periosteum-coated biphasic calcium phosphates were highest, followed by the biphasic calcium phosphates group and the control group at postoperative 4 and 8 weeks (P < 0.05). Histological examination showed that the group treated with tissue-engineered periosteum-coated biphasic calcium phosphates had more osteoblasts, compared with the other two groups. In conclusion, the biphasic calcium phosphate scaffold coated by the tissue-engineered periosteum that is constructed by mesenchymal stem cells from peripheral blood and procine small intestinal submucosa is feasible to repair bone defects.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Stents, Hydroxyapatites

CLC Number:

R318

Xing Fei, Peng Jing, Chen Long, Peng Kun, Li Lang, Xiang Zhou.

Tissue-engineered periosteum-coated biphasic calcium phosphate scaffold repairs bone defects in rabbits

[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(47): 7006-7013.

Figures/Tables 6

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