Biological property of a novel bone cement composited by quaternary ammonium salt chitosan nanoparticles

doi:10.3969/j.issn.2095-4344.2016.47.003

Abstract

Abstract:

BACKGROUND: Postoperative infection in patients underlying total knee arthroplasty is a long-standing puzzle. Current bone cement with antibiotics cannot effectively treat kidney function deficiency and bacterial resistance.
OBJECTIVE: To develop a novel kind of bone cement prepared by quaternary ammonium salt chitosan nanoparticles.
METHODS: There were three groups including QCSNP-15, Palacos R and Palacos R+G groups. Morphology of the bone cement in each group was observed using scanning electron microscope, the setting time of bone cement was measured, and the in vitro compression strength, cytotoxicity and antibacterial activity tests were performed.
RESULTS AND CONCLUSION: Scanning electron microscope observed that QCSNP-15 was made of quaternary ammonium salt chitosan nanoparticles and methyl methacrylate copolymer, and these nanoparticles distributed onto the material surface. The setting time of QCSNP-15 was longer than that of the Palacos R+G bone cement. The compressive strength of QCSNP-15 was significantly lower than that of the Palacos R bone cement (P < 0.05), but was larger than 70 MPa stipulated by ISO 5833. Osteoblasts MC3T3-E1adhered well on the QCSNP-15, and pseudopodia fully expanded. Similar findings were observed on the Palacos R+G bone cement. The attachment rate of osteoblasts of the QCSNP-15 at 3 hours was significantly lower than that of the Palacos R+G bone cement (P < 0.05). The antibacterial activity did not significantly differ between QCSNP-15 and Palacos R+G bone cements, even after 2-week immersion in the PBS. These findings suggest that QCSNP-15 exhibits appropriate setting time, good biomechanical property and antibacterial activity in vitro with no obvious cytotoxicity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Biocompatible Materials, Knee Joint, Chitosan

CLC Number:

R318

He Qiang, Ma Jian-bing, Sun Xiang-xiang, Zhao Guang-hui.

Biological property of a novel bone cement composited by quaternary ammonium salt chitosan nanoparticles

[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(47): 7014-7020.

Figures/Tables 6

References

[1]Bosco J, Bookman J, Slover J, et al. Principles of antibiotic prophylaxis in total joint arthroplasty: current concepts. Instr Course Lect. 2016;65:467-475.
[2]Soares D, Leite P, Barreira P, et al. Antibiotic-loaded bone cement in total joint arthroplasty. Acta Orthop Belg. 2015;81(2):184-190.
[3]Conway JD, Hlad LM, Bark SE. Antibiotic cement-coated plates for management of infected fractures. Am J Orthop. 2015;44(2):E49-E53.
[4]Dzyuba GG, Reznik LB, Erofeev SA, et al. Efficiency of local cement reinforcing antibacterial implants in surgical treatment of long bones chronic osteomyelitis. Khirurgiia. 2016;(5):31-36.
[5]James A, Larson T. Acute renal failure after high-dose antibiotic bone cement: case report and review of the literature. Renal Failure. 2015;37(6):1061-1066.
[6]Hinarejos P, Guirro P, Puig-Verdie L, et al. Use of antibiotic-loaded cement in total knee arthroplasty. World J Orthop. 2015;6(11):877-885.
[7]Dueland R, Spadaro JA, Rahn BA. Silver antibacterial bone cement. Comparison with gentamicin in experimental osteomyelitis. Clin Orthop Relat Res. 1982;(169):264-268.
[8]Miola M, Fucale G, Maina G, et al. Antibacterial and bioactive composite bone cements containing surface silver-doped glass particles. Biomed Materi(Bristol, England). 2015;10(5):055014.
[9]Pauksch L, Hartmann S, Szalay G, et al. In vitro assessment of nanosilver-functionalized PMMA bone cement on primary human mesenchymal stem cells and osteoblasts. PLoS One. 2014;9(12):e114740.
[10]Lyutakov O, Goncharova I, Rimpelova S, et al. Silver release and antimicrobial properties of PMMA films doped with silver ions, nano-particles and complexes. Mater Sci Eng C Mater Biol Appl. 2015;49:534-540.
[11]Rau JV, Fosca M, Graziani V, et al. Silver-doped calcium phosphate bone cements with antibacterial properties. J Funct Biomater. 2016.
[12]Yin W, Li W, Rubenstein DA, et al. Biocompatible and target specific hydrophobically modified glycol chitosan nanoparticles. Biointerphases. 2016;11(4):04b301.
[13]Sarhan WA, Azzazy HM, El-Sherbiny IM. Honey/chitosan nanofiber wound dressing enriched with allium sativum and cleome droserifolia: enhanced antimicrobial and wound healing activity. ACS Appl Mater Interfaces. 2016;8(10):6379-6390.
[14]彭兆祥,郑慷,沈延东,等.关节假体表面壳聚糖季铵盐涂层的抗感染性能研究[J].中华关节外科杂志(电子版), 2014, 8(1):66-71.
[15]Subhapradha N, Shanmugam A. Fabrication of beta-chitosan nanoparticles and its anticancer potential against human hepatoma cells. Int J Biol Macromol. 2016;94(Pt A):194-201.
[16]Jang Y, Je YT, Yun CW, et al. Chitosan dosage regimen to trap fecal oil excretion after peroral lipase inhibitor administration in mice. Int J Biol Macromol. 2016.
[17]Abdelgawad AM, El-Naggar ME, Hudson SM, et al. Fabrication and characterization of bactericidal thiol-chitosan and chitosan iodoacetamide nanofibres. Int J Biol Macromol. 2016;94(Pt A):96-105.
[18]He Q, Chen H, Huang L, et al. Porous surface modified bioactive bone cement for enhanced bone bonding. PLoS One. 2012;7(8):e42525.
[19]Jiang S, Ma BC, Reinholz J, et al. Efficient nanofibrous membranes for antibacterial wound dressing and UV protection. ACS Appl Mater Interfaces. 2016.
[20]Whiteside LA, Roy ME, Nayfeh TA. Intra-articular infusion: a direct approach to treatment of infected total knee arthroplasty. Bone Joint J. 2016;98-b(1 Suppl A): 31-36.
[21]McGlothan KR, Gosmanova EO. A case report of acute interstitial nephritis associated with antibiotic-impregnated orthopedic bone-cement spacer. Tenn Med. 2012;105(9):37-40.
[22]Pinto DC, Pace ED. A silver-stain modification of standard histological slide preparation for use in anthropology analyses. J Forensic Sci. 2015;60(2): 391-398.