Effect of transplantation of bone marrow mesenchymal stem cells on gastric cancer cell line SGC7901 in a rat model of gastric cancer

doi:10.3969/j.issn.2095-4344.2016.41.012

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells can migrate into tumor tissues, as reported in recent studies.
OBJECTIVE: To investigate the tropism and effect of transplantation of bone marrow mesenchymal stem cells on proliferation and differentiation of gastric cancer cell line SGC7901 in rats.
METHODS: Gastric cancer models were established in Sprague-Dawley rats by subcutaneous injection of gastric cancer cell line SGC7901, and then, in cell transplantation group, each rat underwent an intraperitoneal injection of 1×107 bone marrow mesenchymal stem cells. After transplantation, the targeting ability of bone marrow mesenchymal stem cells was detected using fluorescent DiI labeling. Cyclin D2 mRNA and protein expressions were measured using real-time PCR and western blot assay, respectively. Apoptosis of gastric cancer cells was observed by in situ terminal labeling method.
RESULTS AND METHODS: Bone marrow mesenchymal stem cells successfully migrated into the gastric cancer site in rats. The expression levels of cyclin D2 mRNA and protein in the cell transplantation group were significantly higher than those in the model group (P < 0.05). It was observed that the number of apoptotic cancer cells was significantly reduced in the cell transplantation group compared with the model group (P < 0.05). These findings indicate that bone marrow mesenchymal stem cells have the ability to migrate into the tumor sites, thereby promoting the proliferation of gastric cancer cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Stomach Neoplasms, Cyclin D, Tissue Engineering

CLC Number:

R394.2

Chen Chao, Li Hai-tao . Effect of transplantation of bone marrow mesenchymal stem cells on gastric cancer cell line SGC7901 in a rat model of gastric cancer[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(41): 6158-6163.

Figures/Tables 1

References

[1] Parkin DM, Bray F, Ferlay J, et al. Global cancer statistics, 2002.CA Cancer J Clin. 2005;55(2):74-108.
[2] 吴春晓,郑莹,鲍萍萍,等.上海市胃癌发病流行现况与时间趋势分析[J].外科理论与实践, 2008,13(1):24-29.
[3] Kamangar F, Dores GM, Anderson WF. Patterns of cancer incidence, mortality, and prevalence across five continents: defining priorities to reduce cancer disparities in different geographic regions of the world. J Clin Oncol. 2006;24(14):2137-2150.
[4] 谢余澄,杨亚丽,孙涛,等.骨髓间充质干细胞与胃癌的起源[J].临床医学,2012,32(8): 102-104.
[5] Takaishi S, Okumura T, Tu S, et al. Identification of gastric cancer stem cells using the cell surface marker CD44. Stem Cells. 2009;27(5):1006-1020.
[6] Houghton J, Stoicov C, Nomura S, et al. Gastric cancer originating from bone marrow-derived cells. Science. 2004;306(5701):1568-1571.
[7] Marx J. Cancer research. Inflammation and cancer: the link grows stronger. Science. 2004;306(5698): 966-968.
[8] Marx J. Medicine. Bone marrow cells: the source of gastric cancer. Science. 2004;306(5701):1455-1457.
[9] Anzalone R, Lo Iacono M, Loria T, et al. Wharton's jelly mesenchymal stem cells as candidates for beta cells regeneration: extending the differentiative and immunomodulatory benefits of adult mesenchymal stem cells for the treatment of type 1 diabetes. Stem Cell Rev. 2011;7(2):342-363.
[10] Volarevic V, Arsenijevic N, Lukic ML, et al. Concise review: Mesenchymal stem cell treatment of the complications of diabetes mellitus. Stem Cells. 2011; 29(1):5-10.
[11] Tang Y, Gan X, Cheheltani R, et al. Targeted delivery of vascular endothelial growth factor improves stem cell therapy in a rat myocardial infarction model. Nanomedicine. 2014;10(8):1711-1718.
[12] Humphreys BD, Bonventre JV. Mesenchymal stem cells in acute kidney injury. Annu Rev Med. 2008;59: 311-325.
[13] O'Loughlin A, Kulkarni M, Creane M, et al. Topical administration of allogeneic mesenchymal stromal cells seeded in a collagen scaffold augments wound healing and increases angiogenesis in the diabetic rabbit ulcer. Diabetes. 2013;62(7):2588-2594.
[14] Zhang G, Zhou J, Fan Q, et al. Arterial-venous endothelial cell fate is related to vascular endothelial growth factor and Notch status during human bone mesenchymal stem cell differentiation. FEBS Lett. 2008;582(19):2957-2964.
[15] 陈晓宇,黄陈,裘正军.早期胃癌的治疗现状与进展[J].现代生物医学进展,2015, 15(12): 2352-2354.
[16] 李涛,梁美霞,冯道夫,等.进展期胃癌综合治疗分析[J].首都医药, 2014, 21(6): 25-27.
[17] 徐学新,张炜.晚期胃癌的治疗进展[J].中国肿瘤临床与康复,2012, 18(6): 574-576.
[18] 王凯,李玉明,张明凯,等.人胃癌组织中间充质干细胞对胃癌细胞株SGC-7901增殖与侵袭能力的影响[J].中华实验外科杂志, 2014, 31(3): 480-482.
[19] Roorda BD, ter Elst A, Kamps WA, et al. Bone marrow-derived cells and tumor growth: contribution of bone marrow-derived cells to tumor micro-environments with special focus on mesenchymal stem cells. Crit Rev Oncol Hematol. 2009;69(3):187-198.
[20] 赵宝成,王振军,毛伟征,等.脐血间充质干细胞靶向胃癌移植瘤的实验研究[J].肿瘤研究与临床,2011, 23(1): 4-7.
[21] 申晶.骨髓源间充质干细胞输注促进STZ诱导的糖尿病大鼠胰腺内 α 细胞向 β 细胞的转变:糖尿病治疗的新模式[D].北京:中国人民解放军医学院, 2013.
[22] 祝荫.慢病毒载体介导NK4基因修饰骨髓间充质干细胞靶向治疗胃癌的实验研究[D].南昌:南昌大学,2010.
[23] 赵宝成.人脐血间质干细胞趋向胃癌种植瘤及其分子机制的实验研究[D] 青岛:青岛大学, 2008.
[24] 郭彤,王薇,张君,等.骨髓间充质干细胞移植治疗眼表损害的初步实验研究[J].中华眼科杂志,2006,42(3):246-250.
[25] 智晓东,吕刚.大鼠骨髓间充质干细胞和许旺细胞联合移植治疗脊髓损伤[J].中国组织工程研究与临床康复,2008, 12(16): 3015-3018.
[26] 汤庆,何小洪,向邦德,等.人脐静脉血管内皮细胞培养,冻存,复苏与鉴定[J].广州医学院学报, 2006, 34(2):60-63.
[27] 张治金,郭林,赵德伟.兔骨髓间充质干细胞与羊膜的共培养[J].中国组织工程研究,2012,16(27):4959-4962.
[28] 赵聃,李妍,周建博,等.大鼠Thy-1肾炎增殖病变及 sublytic C5b-9致其肾小球系膜细胞增生的实验研究[J].南京医科大学学报, 2012, 12(10):1343-1350.
[29] 赵蕾,朱翠敏,张志华,等.丙戊酸钠逆转Kasumi-1白血病细胞株AML1-ETO融合蛋白转录抑制的作用[J].中国实验血液学杂志, 2009,17(2):363-367.
[30] 郭晓,潘崚. ERK阻滞剂U0126对白血病细胞K562细胞周期的作用及机制[J].第三军医大学学报, 2008, 30(5): 393-395.
[31] 屈建强,杨涛,周乐. p57kip2和cyclin D2在人脑胶质瘤中的表达和临床意义[J].第四军医大学学报, 2007, 28(20): 1878-1880.
[32] 郑倩,刘红,刘华,等.果糖二磷酸钠对2型糖尿病大鼠胰岛内质网应激时CHOP和JNK 表达及胰岛细胞凋亡的影响[J].中国病理生理杂志, 2012, 28(4): 733-737.
[33] 王共先,汪泱,胡红林,等.骨髓间充质干细胞向前列腺癌趋向转移的研究[J].中华实验外科杂志, 2006, 23(5): 582-584.
[34] 宋向明,赵瑜,田长富.肿瘤基因治疗的病毒载体研究进展[J].医学综述,2014,20(6): 1006-1009.
[35] Song H, Hogdall E, Ramus SJ, et al. Effects of common germ-line genetic variation in cell cycle genes on ovarian cancer survival. Clin Cancer Res. 2008; 14(4):1090-1095.
[36] 顾立志.间充质干细胞对胃癌SGC7901细胞增殖的影响及机制的实验研究[D].徐州:徐州医学院, 2009.
[37] Sun T, Sun BC, Ni CS, et al. Pilot study on the interaction between B16 melanoma cell-line and bone-marrow derived mesenchymal stem cells. Cancer Lett. 2008;263(1):35-43.
[38] 陈军,徐祗顺,赵海峰,等.骨髓间充质干细胞在兔肿瘤组织中的分布与分化[J].中华医学杂志, 2007,87(33):2361- 2364.
[39] Karnoub AE, Dash AB, Vo AP, et al. Mesenchymal stem cells within tumour stroma promote breast cancer metastasis. Nature. 2007;449(7162):557-563.