Role of adipose-derived stem cells in the fat transplantation

doi:10.3969/j.issn.2095-4344.2016.41.004

Abstract

Abstract:

BACKGROUND: There are a lot of adipose-derived stem cells in the vascular stroma. These cells are shown to play a very important role in the fat granule transplantation.
OBJECTIVE: To explore the role of adipose-derived stem cells in the fat granule transplantation.
METHODS: Normal adipose tissues were obtained from 10 male BALB/C mice, SPF grade. Adipose-derived stem cells and fat granules were extracted from the abdominal fat tissues. Another 24 nude mice acted as recipients and were assigned into control, fat granule transplantation or mixed transplantation (adipose-derived stem cells+fat granules) groups. In the latter two groups, fat granule suspension and suspension of fat granules and adipose-derived stem cells were injected into the shoulder of rats, respectively. In the control group, the same volume of cell medium was injected. Four weeks later, separated plasma and grafts were taken out for indicator measurement.
RESULTS AND CONCLUSION: Compared with the fat granule transplantation group, the mixed transplantation could remarkably increase the weight of grafts, while reduce the absorption of grafted fat tissues (P < 0.01). After transplantation, the highest level of vascular endothelial growth factor in the plasma was obtained in the mixed transplantation group followed by fat granule transplantation group and control group (P < 0.01). Level of basic fibroblast growth factor and microvessel density were significantly higher in the mixed transplantation group than the fat granule transplantation group (P < 0.01). Better cell morphology and higher number of fat droplets were found in the mixed transplantation group compared with the fat granule transplantation group. All these results indicate that adipose-derived stem cell transplantation can remarkably promote the expression of basic fibroblast growth factor, improve graft microcirculation, and improve morphology and function of fat granules.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Transplantation, Vessels, Tissue Engineering

CLC Number:

R394.2

Zhao Xue-lian, Zhang Chun-li, Su Xiao-guang, Zhang Zhuo-nan, Han Peng, Zhang Jie, Wang Yan-ling, Zhang Chui. Role of adipose-derived stem cells in the fat transplantation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(41): 6105-6111.

Figures/Tables 2

2.1 实验动物数量分析实验选用受体裸鼠24只，分为3组，实验过程中无脱失，全部进入结果分析。 2.2 移植后裸鼠一般状况在实验观察期间动物各项活动和状态较为良好，未出现较严重的不良情况产生，总体动物饮食，活动受移植物的影响较小。 2.3 脂肪干细胞和颗粒脂肪细胞形态观察见图1。实验中发现脂肪干细胞在分离之后，细胞呈现长梭形如图1A所示，在培养传代之后逐渐转变为梭形细胞；颗粒脂肪分离之后，细胞呈现椭圆形的细胞形态(图1B)，在细胞周围散布很多脂肪油滴小点，细胞形态多不相同。 2.4 移植后动物体质量和移植物的质量变化所有动物称质量数据均为采取禁食措施，各项数据均能真实反映动物的体质量变化。由表1可以发现实验进行过程中动物的整体体质量有一定的下降趋势，其中2个移植组相对于对照组体质量出现一定程度的下降，无统计学意义。实验结束时，取出移植物的质量相差较大，颗粒脂肪移植组相比对照组有较大的差异，差异有显著性意义(P < 0.01)。混合移植组的移植物质量明显高于颗粒脂肪移植组，并且差异有显著性意义(P < 0.01)。 2.5 血浆中血管内皮生长因子和碱性成纤维细胞生长因子的变化研究测定血浆中血管内皮生长因子、碱性成纤维细胞生长因子的水平，采用ELISA的试验方法测定血浆中微量生长因子水平，相关数据见表2。由表2可以发现，移植后动物血浆中血管内皮生长因子和碱性成纤维细胞生长因子均明显高于对照组(P < 0.05)，其中混合移植组相对于颗粒脂肪移植组和对照组均能显著性的提高血浆中血管内皮生长因子水平，差异有显著性意义(P < 0.01)；另外混合移植组与颗粒脂肪移植组比较，差异有显著性意义(P < 0.01)。 2.6 微血管密度的水平见表3。由表3可以看出动物在移植后混合移植组移植物中微血管密度明显高于颗粒脂肪移植组，差异有显著性意义(P < 0.01)，微血管密度上升提示脂肪干细胞移植能够显著性增加脂肪组织中微血管的数目，改善移植物的营养供应，提升脂肪细胞的存活率。 2.7 苏木精-伊红染色和油红O染色观察裸鼠移植物脂肪组织周围的油滴数目及细胞形态结果见图2。由图2可以发现油红O染色之后动物的脂肪组织中的油滴数目呈现明显的不同，其中混合移植组明显的增加了脂肪组织周围的油滴数目及颜色，提示动物脂肪细胞的功能较为完好。苏木精-伊红染色后能够发现细胞形态发生了一定的转变，颗粒脂肪移植组的细胞边界模糊，细胞不规则排列，混合移植组细胞明显清晰可见，细胞形态要好于颗粒脂肪移植组。"

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