Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (36): 5364-5370.doi: 10.3969/j.issn.2095-4344.2016.36.007

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Variation of blood biochemical indices in liver cirrhosis rats after adipose-derived mesenchymal stem cell transplantation

Han Hai-yan1, Song Wen-guang2   

  1. 1Department of Clinical Laboratory, 2Reproductive Medicine Center, Affiliated Hospital of Logistics University of CAPF, Tianjin 300162, China
  • Revised:2016-07-27 Online:2016-09-02 Published:2016-09-02
  • About author:Han Hai-yan, Laboratory technician, Department of Clinical Laboratory, Affiliated Hospital of Logistics University of CAPF, Tianjin 300162, China Song Wen-guang, Technologist-in-charge, Reproductive Medicine Center, Affiliated Hospital of Logistics University of CAPF, Tianjin 300162, China Han Hai-yan and Song Wen-guang contributed equally to this work.

Abstract:

BACKGROUND: Stem cell transplantation is a promising treatment of advanced liver disease, and adipose-derived mesenchymal stem cells have become another kind of popular cells following bone marrow mesenchymal stem cells.
OBJECTIVE: To investigate the influence of adipose-derived mesenchymal stem cell transplantation on blood biochemical indices of liver cirrhosis rats.
METHODS: Sixty rats were equally randomized into normal control, model and cell transplantation groups. Model rats of liver cirrhosis were made in the latter two groups through intragastric administration of carbon tetrachloride. One week after successful modeling, rats were given intraperitoneal injection of adipose-derived mesenchymal stem cell suspension in the cell transplantation group, and given normal saline in the other two groups.
RESULTS AND CONCLUSION: Compared with the normal control group, the model group showed a significant increase in the levels of alanine aminotransferase, aspartate aminotransferase, total bilirubin, total protein in liver tissues, serum level of malondialdehyde, 15-minute indocyanine green retention rate and degree of hepatic fibrosis, and a significant decrease in serum albumin level, serum albumin/globulin, levels of glutathione peroxidase and cyclic guanosine monophosphate in liver tissues. On the contrary, these indicators were all improved in the cell transplantation group compared with the model group. Moreover, CM-Dil-positive cells were visible in the liver tissue of rats undergoing adipose-derived mesenchymal stem cell transplantation. All these findings indicate that adipose-derived mesenchymal stem cell transplantation can reduce liver cirrhosis in rats by acting on blood biochemistry levels.

 

中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Liver Cirrhosis, Stem Cell Transplantation, Tissue Engineering

CLC Number: