Bone marrow mesenchymal stem cell transplantation improves behavior performance of senile dementia rats

doi:10.3969/j.issn.2095-4344.2016.36.008

Abstract

Abstract:

BACKGROUND: More recently, studies have demonstrated that bone marrow mesenchymal stem cells can be induced in vitro to differentiate into neuron-like cells that are used for in vivo transplantation to repair nerve damage.
OBJECTIVE: To study the effect of bone marrow mesenchymal stem cell transplantation on learning and memory ability of senile dementia rats.
METHODS: Thirty male Sprague-Dawley rats were randomly divided into three groups: normal control group, stem cell therapy group and model control group. Rats in the latter two groups were used to establish animal models of senile dementia by intracranial injection of β-amyloid 1-40. Three weeks after modeling, rats were given bilateral hippocampal injection of induced bone marrow mesenchymal stem cell suspension in the stem cell therapy group, whereas no treatment was given in the normal control and model control groups. Morris water maze test was used to detect learning and memory ability of rats, and rat’s brain tissues were detected pathologically using hematoxylin-eosin staining.
RESULTS AND CONCLUSION: After modeling, the escape latency was higher and the cross-platform frequency was lower in the model control group compared with the normal control group. After cell transplantation, the escape latency and cross-platform frequency were gradually shortened and increased with time, respectively. Compared with the model control group, the learning and memory abilities of rats were improved in the stem cell therapy group. The brain tissues were relatively intact in structure and exhibited less cell degeneration and necrosis in the stem cell therapy group compared with the model control group. To conclude, bone marrow mesenchymal stem cell transplantation exerts certain therapeutic effects on senile dementia by effectively improving the learning and memory ability.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Alzheimer Disease, Tissue Engineering

CLC Number:

R394.2

Zhang Ying, Guo Jian-hua, Yan Hong-juan, Luo Qiu-hua, Li Xin-ping, Guo Wei, Wang Hai-xia . Bone marrow mesenchymal stem cell transplantation improves behavior performance of senile dementia rats[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(36): 5371-5377.

Figures/Tables 3

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