Bone marrow mesenchymal stem cells for repairing acute respiratory distress syndrome

doi:10.3969/j.issn.2095-4344.2016.32.012

Abstract

Abstract:

BACKGROUND: To date little is reported clinically on bone marrow mesenchymal stem cells (BMSCs) for acute respiratory distress syndrome (ARDS).
OBJECTIVE: To study the effect of BMSCs in ARDS rats.
METHODS: Sixty-three Sprague-Dawley rats were randomly assigned into normal control group, model and BMSCs groups (n=21 per group), followed by establishment of ARDS models. After awakening, rats in the model group were given no treatment, while those in the BMSCs group given tail vein injection of fluorouracil-labeled BMSCs. Then, pathological observation of the lung tissue was conducted using hematoxylin-eosin staining, and ELISA method was employed to detect interleukin-1β level in the rat lung tissue homogenates. Repair effects on lung injury were compared between two groups.
RESULTS AND CONCLUSION: Hematoxylin-eosin staining showed that inflammation, edema and congestion in the rat lung tissue were vanished gradually, but no consolidation was found in the BMSCs group, while there was visible edema and congestion in the lung tissue of rats in the model group. Compared with the model group, pulmonary edema and interleukin-1β level in the lung tissue were significantly reduced in the BMSCs group (P < 0.05). Therefore, the whole bone marrow culture method is suitable to obtain BMSCs that have desired effects on ARDS in rats. In ARDS rats, due to inhalation of lipopolysaccharide, BMSCs can be directly involved in lung epithelial repair by reducing the release of inflammatory factors.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Mesenchymal Stem Cells, Respiratory Distress Syndrome, Adult, Tissue Engineering

CLC Number:

R394.2

Zhang Lei. Bone marrow mesenchymal stem cells for repairing acute respiratory distress syndrome[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(32): 4792-4797.

Figures/Tables 1

2.1 实验动物数量分析实验选用SD大鼠63只，全部进行结果分析数量，中途无脱失。 2.2 大鼠骨髓间充质干细胞培养与鉴定采集的骨髓间充质干细胞培养7 d后细胞开始贴壁生长，细胞体积较小，成梭形，并且其形态与成纤细胞十分类似，细胞核较大，核浆较大，细胞聚集生长，见图1A。第2代骨髓间充质干细胞分布相对比较均匀，见图1B。 2.3 骨髓间充质干细胞鉴定通过免疫荧光法对大鼠骨髓间充质干细胞进行鉴定，结果显示：骨髓间充质干细胞CD29、CD44呈阳性，CD34和CD45为阴性。 2.4 大鼠骨髓间充质干细胞传代生长情况骨髓间充质干细胞在传代生长过程中随传代次数的不断增加，细胞增殖能力出现下降趋势，且每次传代经历两三天，经2-5 d后经历对数增殖期而进入平台期，见表1，图2。 2.5 各组大鼠大体观察 3组大鼠均取得手术成功，损伤对照组6只出现呼吸困难，2只不活动，3只不进食；骨髓间充质干细胞组1只呼吸困难，1只不活动，均能按时进食，对照组大鼠呼吸正常，未见异常。3组大鼠大体观察差异有显著性意义(P < 0.05)。 2.6 各组大鼠肺组织观察 2个月后处死大鼠，对肺部组织进行切片苏木精-伊红染色结果显示：骨髓间充质干细胞组大鼠肺部炎症细胞消退，肺部组织水肿、充血开始消退，并未发生实变情况见图3A；损伤对照组肺部组织炎症变化明显，存在明显的水肿和充血情况，见图3B；正常对照组大鼠肺部组织见图3C。 2.7 各组大鼠氧合指数、肺水肿指数、匀浆白细胞介素1β含量比较骨髓间充质干细胞组氧合指数显著增高，肺水肿指数、肺组织匀浆白细胞介素1β水平，显著低于损伤对照组(P < 0.05)，损伤组显著高于正常对照组(P < 0.05)，见表2。"

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