Different culture methods for human umbilical cord mesenchymal stem cells

doi:10.3969/j.issn.2095-4344.2016.28.005

Abstract

Abstract:

BACKGROUND: Umbilical cord stem cells mainly derive from full-term infants, and common culture methods include tissue-attached method and trypsin-digestion mehod. However, effects of different culture methods on the separation of umbilical cord mesenchymal stem cells remain many disputes.
OBJECTIVE: To observe the effects of different culture methods on umbilical cord mesenchymal stem cells.
METHODS: Umbilical cords of 30 healthy full-term and caesarean delivery infants were selected, and cultured using tissue-attached method or trypsin-digestion method to isloate and culture human umbilical cord mesenchymal stem cells. Meanwhile, cell growth was measured by flow cytometry.
RESULTS AND CONCLUSION: The fusiform-shaped cells began to separate from the umbilical cord tissue that was primary cultured using tissue-attached method, and 10 days later, the cell fusion reached 80%; after the umbilical cord was cultured using collagenase-trypsin digestion for 5 days, a small amount of adherent cells with different shapes appeared, and the fiber-like cells reached 80% of confluence until 2-week culture. There was no significant difference in the growth of umbilical cord mesenchymal stem cells cultured by different culture methods (P > 0.05). Moreover, cells cultured by two methods were all positive for CD13, CD29, CD44 and CD105. These results demonstrate that human umbilical cord mesenchymal stem cells exhibit a high success rate in primary culture using tissue-attached method, which is superior to the trypsin-digestion method.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Engineering, Umbilical Cord, Stem Cells

CLC Number:

R394.2

Liu Feng, Jiang Xiang-lin, Liu Ming-ming. Different culture methods for human umbilical cord mesenchymal stem cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(28): 4136-4141.

Figures/Tables 1

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