Three-dimensionally printed porous beta-tricalcium phosphate scaffold loading poly(lactic-co-glycolic acid)/anti-tuberculosis drug control-release microspheres: a cytotoxic evaluation

doi:10.3969/j.issn.2095-4344.2016.25.016

Abstract

Abstract:

BACKGROUND: So far there is a lack of reliable biomedical evidence about the effects of three-dimensionally (3D) printed porous β-tricalcium phosphate (β-TCP) scaffold loading poly(lactic-co-glycolic acid) (PLGA)/anti-tuberculosis drug control-release microspheres on the growth and proliferation of cells, especially osteoblasts.

OBJECTIVE: To construct porous β-TCP scaffold loading PLGA/anti-tuberculosis drug control-release microspheres by 3D printing technology and to detect its cytotoxicity.

METHODS: Twenty porous β-TCP scaffolds whose aperture was 400 μm were prepared by 3D printing technology. Ten of these scaffolds were randomly selected for loading PLGA/anti-tuberculosis drug control-release microspheres, and the others were without any drugs. Then the extracts from two groups were cultured with osteoblasts for 72 hours. Afterwards, cell morphology was observed by inverted phase contrast microscope, and the absorbance value was detected using cell counting kit-8 assay. Besides, the relative growth rate of osteoblasts was calculated to evaluate the cytotoxicity of the scaffold.

RESULTS AND CONCLUSION: The drug-loaded scaffold exhibited with moderate size, regular structure and uniform pores. Within 72 hours of culture in the extracts from the drug-loaded scaffold, elongated or fusiform osteoblasts appeared, with less karyopycnosis. Moreover, the drug-loaded scaffold showed slight cytotoxicity, which was classified as grade 1. In conclusion, the 3D-pinted porous β-TCP scaffold loading PLGA/anti-tuberculosis drug control-release microspheres exhibits no obvious cytotoxicity.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Calcium Phosphates, Antitubercular Agents, Tissue Engineering

CLC Number:

R318

Meng Lei, Zhen Ping, Liang Xiao-yan. Three-dimensionally printed porous beta-tricalcium phosphate scaffold loading poly(lactic-co-glycolic acid)/anti-tuberculosis drug control-release microspheres: a cytotoxic evaluation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(25): 3750-3756.

Figures/Tables 7

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