Nano-cellulose protein versus acellular matrix in oral mucosa repair

doi:10.3969/j.issn.2095-4344.2016.21.009

Abstract

Abstract:

BACKGROUND: The sensitivity and mucus secretion of the oral mucosa make oral soft tissues difficult to repair, so patients cannot achieve satisfactory outcomes after treatment. Nano-cellulose protein mainly composed of glycine, alanine and serine has good histocompatibility. However, there is a lack of comparative study about the effect of nano-cellulose protein and acellular matrix in oral mucosa repair, and the clinical effects of the two materials are still under discussion.

OBJECTIVE: To investigate the effects of nano-cellulose protein versus acellular matrix in oral mucosa repair.

METHODS: Oral mucosa defect models were prepared in rats, and these rat models were randomly divided into four groups: oral mucosa defects were repaired by vaseline (control group), nano-cellulose protein, bovine skin acellular matrix and human skin acellular matrix, respectively. Repair effects were compared among different materials within 2 months after surgery.

RESULTS AND CONCLUSION: The diameter of oral mucosa defect measured using a vernier caliper, had no significant differences among groups at 1 day after surgery (P > 0.05); the diameter of oral mucosa defect in the nano-cellulose protein group was significantly lower than that in the other groups at 3, 5 and 7 days after surgery (P < 0.05); the diameter of oral mucosa defect in the bovine and human skin acellular matrix groups was significantly lower than that in the control group at 5 and 7 days after surgery (P < 0.05). Morphological observation of the oral mucosa under light microscope showed: the number of newborn capillary endothelial cells in the defect region had no significant differences among nano-cellulose protein, bovine acellular matrix and human acellular matrix groups at 1, 3, 5 and 7 weeks after surgery (P > 0.05); but there were significant differences in the number of newborn capillary endothelial cells between the control group and the other three groups (P < 0.05). Furthermore, at 21 days after surgery, closely aligned and thicker new epithelial tissue could be found in the nano-cellulose protein group; in the bovine acellular matrix group, the defect region was repaired well, new epithelial tissue appeared and the number of inflammatory cells decreased; in the human acellular matrix group, inflammatory cells appeared obviously, and new epithelial tissue formed with the normal thickness. In contrast, abundant inflammatory cells and thinner epithelial tissues appeared in the control group. To conclude, both nano-cellulose protein and acellular matrix can accelerate wound healing by promoting oral mucosal epithelial hyperplasia.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biocompatible Materials, Tissue Engineering, Soft Tissue Injuries

CLC Number:

R318

Wang Zhong-chao, Fan Li-yuan, Cai Wei, Jiang Jun-qiang. Nano-cellulose protein versus acellular matrix in oral mucosa repair[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(21): 3104-3109.

Figures/Tables 3

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