Constructing tissue-engineered bladder by vascular endothelial growth factor nanoparticle-bacterial cellulose composite scaffold with various kinds of cells

doi:10.3969/j.issn.2095-4344.2016.21.007

Abstract

Abstract:

BACKGROUND: Traditional bladder repair methods have many problems such as damage to normal organ function and many postoperative complications. Tissue engineering technology provides a new way for bladder repair.

OBJECTIVE: To explore the feasibility of constructing tissue-engineered bladder with vascular endothelial growth factor (VEGF) nanoparticle-bacterial cellulose (BC) composite scaffold with rabbit lingual epithelial cells and tongue muscle cells.

METHODS: Rabbit lingual epithelial cells and muscle cells were successively implanted onto the BC scaffold (control group) and VEGF-BC scaffold (experimental group). Six rabbits were taken to make bladder defect models and randomized into two groups: experimental group implanted with VEGF-BC scaffold carrying autologous lingual epithelial cells and tongue muscle cells, and control group implanted with BC scaffold carrying autologous lingual epithelial cells and tongue muscle cells. Specimens were taken from the two groups for urographic evaluation and histological examination at 3 months after implantation. Meanwhile, the urodynamic tests were performed.

RESULTS AND CONCLUSION: The experimental group showed the relatively complete bladder, and the control group showed a small-area filling defect of the bladder. The maximum bladder capacity and bladder compliance in both two groups were decreased after implantation, especially significantly in the control group (P < 0.05). In the control group, it failed to build a complete epithelial cell layer, and the muscle layer and microvessels were formed a little. In the experimental group, the complete epithelial cell layer was formed, and a larger amount of muscle layers and capillaries appeared. These findings indicate that the VEGF-BC scaffold carrying lingual epithelial cells and tongue muscle cells can be used to construct the tissue-engineered bladder.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Cellulose, Urinary Bladder, Epithelial Cells, Muscle Cells, Tissue Engineering

CLC Number:

R318

Zhang Xin-ru, Lu Wen-long, Feng Chao, Lv Xiang-guo, Zhu Wei-dong . Constructing tissue-engineered bladder by vascular endothelial growth factor nanoparticle-bacterial cellulose composite scaffold with various kinds of cells[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(21): 3088-3096.

Figures/Tables 8

References

[1] Oberpenning F,Meng J,Yoo JJ,et al. De novo reconstitution of a functional mammalian urinary bladder by tissue enginnering. Nature Biotechnol. 1999;17:149-155.

[2] Atala A,Bauer SB,Soker S,et al.Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet.2006;367:1241-1246.

[3] Byung SK,Anthony A,James JY,et al. A collagen matrix derived from bladder can be used to engineer smooth muscle tissue.World J Urol. 2008;26:307-314.

[4] Yoo JJ,Meng J,Oberpenning F,et al.Bladder augmentation using allogenic bladder submucosa seeded with cells.Urology. 1998; 51(2):221-225.

[5] Zhu WD,Xu YM,Feng C,et al.Different bladder defects reconstructed with bladder acellular matrix grafts in a rabbit model.Urologe. 2011;50:1420-1425.

[6] Cheng EY,Kropp BP.Urologic tissue engineering with small—intestinal submucosa：potential clinical applications.World J Urol.2000;18:26-30．

[7] Sievert KD,Tanagho EA.Organ specific acellular matrix for reconstruction of the urinary tract.World J Urol.2000;18:19-25．

[8] Kropp BP,Sawyer BD,Shannon H E,et al. Characterization of small intestinal submucosa regenerated canine detrusor: assessment of reinnervation, in vitro compliance and contractiIity.J Uro1.1996;156:599-607．

[9] Anilkumar TV,Vineetha VP,Revi D,et al. Biomaterial properties of cholecyst-derived scaffold recovered by a non-detergent/enzymatic method.J Biomed Mater Res B Appl Biomater. 2014; 102:1506-1516.

[10] Atala A.Tissue engineering of human bladder.Br Med Bull.2011;97:81-104.

[11] Record RD,Hillegonds D,Simmons C,et al.In vivo degradation of 14C-labeled small intestinal submucosa (SIS) when used for urinary bladder repair.Biomaterials.2001; 22:2653-2659.

[12] Bolland F,Korossis S,Wilshaw SP,et al. Development and characterisation of a full-thickness acellular porcine bladder matrix for tissue engineering. Biomaterials. 2007;28:1061-1070.

[13] Zhang Y,Frimberger D,Cheng EY,et al.Challenges in a larger bladder replacement with cell-seeded and unseeded small intestinal submucosa grafts in a subtotal cystectomy model.BJU Int. 2006;98:1100-1105.

[14] Pokrywczynska M,Jundzill A,Bodnar M,et al.Do mesenchymal stem cells modulate the milieu of reconstructed bladder wall? Arch Immunol Ther Exp (Warsz).2013;61:483-493.

[15] Pokrywczynska M,Adamowicz J,Sharma AK,et al.Human urinary bladder regeneration through tissue engineering -an analysis of 131 clinical cases. Exp Biol Med Maywood NJ.2013;239:264-271.

[16] Schaefer M,Kaiser A,Stehr M,et al.Bladder augmentation with small intestinal submucosa leads to unsatisfactory long-term results. J Pediatr Urol. 2013;9:878-883.

[17] Atala A,Bauer SB,Soker S,et al.Tissue-engineered autologous bladders for patients needing cystoplasty. Lancet.2006;15:1241-1246.

[18] Petrovic V,Stankovic J,Stefanovic V.Tissue engineering of the urinary bladder: current concepts and future perspectives.Scientific World J. 2011;11: 1479-1488.

[19] Lam Van Ba O, Aharony S, Loutochin O, et al. Bladder tissue engineering: A literature review.Adv Drug Deliv Rev. 2015; 82-83:31-37.

[20] Petersen N,Gatenholm P.Bacterial cellulose-based materials and medical devices: current state and perspectives.Appl Microbiol Biotechnol. 2011; 91(5):1277-1286.

[21] Moreira S,Silva NB,Almeida-Lima J,et al.BC nanofibres: in vitro study of genotoxicity and cell proliferation.Toxicol Lett.2009;189(3):235-241.

[22] Dugan JM,Gough JE,Eichhorn SJ,et al.Bacterial cellulose scaffolds and cellulose nanowhiskers for tissue engineering.Nanomedicine(Lond). 2013;8(2): 287-298.

[23] Esguerra M,Fink H,Laschke MW,et al.Intravital fluorescent microscopic evaluation of bacterial cellulose as scaffold for vascular grafts.J Biomed Mater Res A. 2010;93(1):140-149.

[24] Helenius G,Backdahl H,Bodin A,et al.In vivo biocompatibility of bacterial cellulose. J Biomed Mater Res A.2006;76(2):431-438.

[25] Brackmann C,Zaborowska M,Sundberg J,et al.In situ Imaging of Collagen Synthesis by Osteoprogenitor Cells in Microporous Bacterial Cellulose Scaffolds. Tissue Eng Part C Methods. 2012;18(3): 227-234.

[26] Andersson J,Stenhamre H,Bäckdahl H,et al.Behavior of human chondrocytes in engineered porous bacterial bcellulose scaffolds.J Biomed Mater Res A.2010;94(4): 1124-1132.

[27] Sanchavanakit N,Sangrungraungroj W, Kaomongkolgit R, et al.Growth of Human Keratinocytes and Fibroblasts on Bacterial Cellulose Film. Biotechnol Prog.2006;22(4): 1194-1199.

[28] Wippermann J,Schumann D,Klemm D,et al. Preliminary Results of Small Arterial Substitute Performed with a New Cylindrical Biomaterial Composed of Bacterial Cellulose. Eur J Vasc Endovasc Surg.2009;37(5):592-596.

[29] Zhou L,Yang B,Sun C,et al.Coadministration of plateletderived growth factor-BB and vascular endothelial growth factor with bladder acellular matrix enhances smooth muscle regeneration and vascularization for bladder augmentation in a rabbit model.Tissue Eng A.2013;19:264-276.

[30] Youssif M,Shiina H,Urakami S,et al.Effect of vascular endothelial growth factor on regeneration of bladder acellular matrix graft: histologic and functional evaluation. Urology.2005;66:201-207.

[31] Chen BS,Xie H,Zhang SL,et al.Tissue engineering of bladder using vascular endothelial growth factor gene-modified endothelial progenitor cells.Int J Artif Organs.2011; 34:1137-1146.

[32] Loai Y,Yeger H,Coz C,et al.Bladder tissue engineering: tissue regeneration and neovascularization of HA-VEGF-incorporated bladder acellular constructs in mouse and porcine animal models.J Biomed Mater Res A.2010;15: 1205-1215.

[33] Kikuno N,Kawamoto K,Hirata H,et al.Nerve growth factor combined with vascular endothelial growth factor enhances regeneration of bladder acellular matrix graft in spinal cord injury-induced neurogenic rat bladder.BJU Int. 2009;103:1424-1428.

[34] Cartwright L,Farhat WA,Sherman C,et al.Dynamic contrast-enhanced MRI to quantify VEGF- enhanced tissue-engineered bladder graft neovascularization: pilot study. Biomed Mater Res A.2006;77:390-395.

[35] Xiong Q,Lin H,Hua X,et al.A nanomedicine approach to effectively inhibit contracture during bladder acellular matrix allograft-induced bladder regeneration by sustained delivery of vascular endothelial growth factor.Tissue Eng Part A. 2015; 21(1-2):45-52.

[36] Jayo MJ,Jain D,Wagner BJ,et al.Early cellular and stromal responses in regeneration versus repair of a mammalian bladder using autologous cell and biodegradable scaffold technologies.J Urol.2008;180: 392-397.

[37] 朱卫东,徐月敏,冯超,等.脂肪干细胞复合膀胱脱细胞基质促进膀胱再生的实验研究[J].中华泌尿外科杂志, 2012,33(2):111-116.

[38] Sievert KD,Feil G,Renninger M,et al.Tissue engineering and stem cell research in urology for a reconstructive or regenerative treatment approach.Urologe A. 2007;46(9):1224-1230.

[39] Becker C,Jakse G.Stem cells for regeneration of urological structures.Eur Urol. 2007;51(5): 1217-1228.

[40] Polykandriotis E,Arkudas A,Horch RE,et al. Autonomously vascularized cellular constructs in tissue engineering: opening a new perspective for biomedical science. J Cell Mol Med.2007;11(1):6-20.