Distribution and differentiation of embryonic liver stem cells in mice after intrahepatic transplantation

doi:10.3969/j.issn.2095-4344.2016.19.017

Abstract

Abstract:

BACKGROUND: As many factors can lead to liver injury, we attempt to use the “therapeutic liver regeneration” technology in clinical treatment of liver diseases by promoting liver regeneration.
OBJECTIVE: To investigate distribution and differentiation of embryonic liver stem cells in mice after intrahepatic transplantation via a transplantation approach.
METHODS: Liver injury models were prepared in 20 BALB/c mice, and then randomly equivalently assigned into two groups: 70% partial hepatectomy with intrahepatic transplantation with 1×10⁵ embryonic liver stem cells in control group; therapeutic liver regeneration model plus intrahepatic transplantation with 1x10⁵ embryonic liver stem cells in observation group. At 1 and 2 weeks after cell transplantation, the liver parenchyma of mice was observed. And at 2 weeks, both of the two groups underwent confocal immunofluorescence assay. Besides, blood samples of mouse tail vein were collected to detect levels of serum albumin.
RESULTS AND CONCLUSION: At 1 week after cell transplantation, in the liver parenchyma, green fluorescence was sparsely distributed in the two groups, and the distribution density had no significant difference between the two groups; at 2 weeks after cell transplantation, hepatic cord-like structures appeared in the liver parenchyma of two groups, and the green fluorescence distribution in the control group was limited, but significantly expanded in the observation group. At 2 weeks after cell transplantation, positive albumin expression in the liver parenchyma was significantly higher in the observation group than in the control group, and there was no significant difference in levels of serum albumin between two groups (P > 0.05). To conclude, after transplantation of embryonic liver stem cells in the therapeutic liver regeneration model mice hepatocytes can be effectively integrated into the host hepatic plate, differentiate in the liver, and partially trigger the function of hepatocytes.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Stem Cell Transplantation, Cell Differentiation, Liver Regeneration, Tissue Engineering

CLC Number:

R394.2

Jiang Si-feng. Distribution and differentiation of embryonic liver stem cells in mice after intrahepatic transplantation[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(19): 2850-2856.

Figures/Tables 1

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