High-absorbing chitosan dressings for hemostasis and wound healing

doi:10.3969/j.issn.2095-4344.2016.16.015

Abstract

Abstract:

BACKGROUND: High liquid-absorbing property, high-capacity imbibition property and high antibacterial property of chitosan dressings can be promoted by chemical modification.

OBJECTIVE: To observe the effect of high-absorbing chitosan dressings on hemostasis and wound healing.

METHODS: (1) Venule bleeding experiment: symmetry oozing wounds were produced on the rabbit skin, which were respectively covered by high-absorbing chitosan dressings and sterile gauze. Then, hemostatic time and bleeding volume were recorded. (2) Deep II degree burn wound healing experiment: 40 Sprague-Dawley rats were selected to prepare deep II degree burn wounds on the back, and then model rats were randomly divided into two groups: experimental group with high-absorbing chitosan dressings and control group with vaseline gauze. After 3, 7, 14, 21 days, wound tissues were taken and analyzed histopathologically.

RESULTS AND CONCLUSION: (1) Venule bleeding experiment: The bleeding amount and hemostatic time were significantly lower in the high-absorbing chitosan dressing group than the sterile gauze group (P < 0.05, P < 0.01). (2) Deep II degree burn wound healing experiment: the unhealed wound areas were significantly lower in the experimental group than the control group at days 7, 14, 21 after repair (P < 0.05). After 3 days of wound repair, necrosis of the squamous epithelial layer and the structural damage of the hair follicle in the corium layer and skin appendages were found in both two groups. Meanwhile, the infiltration caused by varying numbers of neutrophils and lymphocytes inside the damaged skin tissues was visible. After 21 days of repair, the epithelium healed well in the control group, with a little lymphocyte infiltration and formation of crust on the skin surface; while in the experimental group, the epithelium healed well, with complete squamous epithelial layer and no inflammatory cell infiltration. Thus, these experimental findings reveal that the high-absorbing chitosan dressing has an excellent effect on hemostasis and wound healing.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Chitosan, Biological Dressings, Hemostasis, Tissue Engineering

Mao Jun, Zhou Ying-shan, Wu Ting. High-absorbing chitosan dressings for hemostasis and wound healing[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(16): 2391-2396.

Figures/Tables 3

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