Differential gene expression between Xinjiang Uygur and Han patients with ankylosing spondylitis

doi:10.3969/j.issn.2095-4344.2016.15.015

Abstract

Abstract:

BACKGROUND: Genetic factors, environment, chronic infection, and autoimmune disorders are considered to be involved in the pathogenesis of ankylosing spondylitis. Genetic factors play an important role in the pathogenesis of the disease. Ethnic and regional diversity of differentially expressed genes has become research hotspot because of family aggregation and ethnic diversity of ankylosing spondylitis.

OBJECTIVE: To screen differentially expressed genes in Xinjiang Uygur and Han patients with ankylosing spondylitis by microarray screening and compare differences in gene expressions.

METHODS: Uygur and Han patients with active ankylosing spondylitis in department of rheumatology of our hospital were randomly collected with five patients for each. In addition, three healthy volunteers were selected as controls. RNA from peripheral blood was extracted and used for microarray hybridization after probe preparation to screen differentially expressed genes in ankylosing spondylitis samples and the microarray results were verified by semi-quantitative RT-PCR analysis.

RESULTS AND CONCLUSION: Twenty differentially expressed miRNAs were screened in Uygur and Han patients with active ankylosing spondylitis (P < 0.05). From relationship analysis of target genes and miRNAs, 15 target genes corresponding to the 79 miRNAs involved in human leucocyte antigens and interleukins which linked to human immunity system were found. These findings suggest that differentially expressed genes can be screened from Uygur and Han patients with ankylosing spondylitis.
中国组织工程研究杂志出版内容重点：组织构建；骨细胞；软骨细胞；细胞培养；成纤维细胞；血管内皮细胞；骨质疏松；组织工程

Key words: Tissue Engineering, Heredity, Genes

Liu Zhen-feng, Liang Zhi-quan, Hong Han-gang, Fang Rui, Elijan•Asla, Zhao Jiang. Differential gene expression between Xinjiang Uygur and Han patients with ankylosing spondylitis[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(15): 2233-2240.

Figures/Tables 5

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