Neural stem cell transplantation for treatment of cerebral palsy

doi:10.3969/j.issn.2095-4344.2016.14.017

Abstract

Abstract:

BACKGROUND: Transplanted neural stem cells can survive, proliferate and differentiate into neurons and/or glial cells in the host, thereby promoting partial function recovery in the host.

OBJECTIVE: To study the therapeutic effects of neural stem cell transplantation on cerebral palsy rats.

METHODS: Twenty-four rats were randomly divided into three groups: control group, model group and transplantation group. Animal models of cerebral palsy were made in the latter two groups. One week after modeling, rats in the transplantation group were injected 1 mL stem cell suspension (1×10⁵) via the jugular vein, and rats in the control and model group were given the same volume of normal saline. Toe distance, step length and elevated body swing test in rats were detected, and histopathological changes in the rat brain were observed 3 weeks after transplantation.

RESULTS AND CONCLUSION: In the model group, the toe distance and step length of the front left palm were significantly lower than those of the front right palm (P < 0.05). Compared with the model group, the toe distance and step length in the transplantation and control groups were significantly increased (P < 0.05). In the elevated body swing test, rats in the model group presented with asymmetric swing of the body, but rats in the other two groups exhibited symmetric swing of the body (P < 0.05). Additionally, the ratio of right to left hemispheric areas was significantly higher in the transplantation and control groups compared with the model group (P < 0.05). In conclusion, neural stem cell transplantation via the jugular vein can improve brain function and restore motor function in rats with cerebral palsy.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Neural Stem Cells, Cerebral Palsy, Cell Transplantation, Tissue Engineering

Lou Yuan-jun, Liu Yang, Shan Hai-jun, Cao Cai-hong, Jie Xiao-su. Neural stem cell transplantation for treatment of cerebral palsy[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2092-2097.

Figures/Tables 2

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