Chinese Journal of Tissue Engineering Research ›› 2016, Vol. 20 ›› Issue (14): 2021-2026.doi: 10.3969/j.issn.2095-4344.2016.14.006

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Role and mechanism of gastric cancer stem cells in gastric cancer invasion, metastasis and angiogenesis

Huang Hui, Pan Zhi-jian   

  1. the Affiliated Wuhan Central Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430024, Hubei Province, China
  • Received:2016-02-22 Online:2016-04-01 Published:2016-04-01
  • About author:Huang Hui, Master, Attending physician, the Affiliated Wuhan Central Hospital of Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430024, Hubei Province, China

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Abstract:

BACKGROUND: Although tumor stem cells and their differentiated vascular cells, which are not sensitive to chemotherapy and molecular targeted therapy, reduce overall blood supply of the tumor, these cells facilitate the invasion and metastasis of the tumor, eventually resulting in treatment failure.

OBJECTIVE: To investigate the effect of gastric cancer stem cells in stomach cancer invasion and metastasis as well as its mechanism in angiogenesis.
METHODS: A total of 20 gastric cancer stem cells and 20 normal gastric cancer cells SGC7901 were cultured in the medium with DMEM/F12 (1:1), 2% B27 and 20 μg/L epidermal growth factors or cultured in 10% PBS, respectively. Invasion of gastric cancer stem cells was detected by sphere formation assay, colony formation assay and Transwell invasion assay. Levels of mRNA and protein expression of CD44 and E-cadherin were measured to observe tumor metastasis. Besides, angiogenesis was observed by cell scratch test, Transwell migration assay and ring test.
RESULTS AND CONCLUSION: Gastric cancer cells adhered to the medium wall, and gastric cancer stem cells suspended in the medium, which appeared to be spindle interstitial form. Number of gastric cancer tumor spheres was significantly lower than that of gastric cancer stem cell spheres in the sphere formation assay and colony formation assay (P < 0.05). Number of gastric cancer stem cells through the basement membrane was significantly higher than that of gastric cancer cells (P < 0.05). And the cell migration distance and ring number were significantly increased in the gastric cancer stem cells as compared with the gastric cancer cells (P < 0.05). In conclusion, the invasion, migration and angiogenesis abilities of gastric cancer stem cells are stronger than those of gastric cancer cells, which can further promote the occurrence and progression of gastric cancer. 
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程 

Key words: Neoplastic Stem Cells, Stomach Neoplasms, Molecular Targeted Therapy, Neoplasm Metastasis, Tissue Engineering