Proliferative activity of bone marrow mesenchymal stem cells cultured in vitro: assessment by a CT brain perfusion scan

doi:10.3969/j.issn.2095-4344.2016.14.007

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cell transplantation is a hot spot in the treatment of ischemic cerebrovascular diseases, and CT brain perfusion scan is mostly used to observe the improvement in the blood flow in the ischemic region. However, X-ray irradiation during CT brain perfusion may affect the viability of transplanted bone marrow mesenchymal stem cells, and then influence the therapeutic efficacy.

OBJECTIVE: To investigate the effect of CT brain perfusion dose on the viability of bone marrow mesenchymal stem cells.

METHODS: Passage 5 bone marrow mesenchymal stem cells from Sprague-Dawley rats were selected and randomized into three groups, with 6 tubes in each group. One of the six tubes in each group was randomly selected with no irradiation, and the remaining five tubes in each group were subjected to CT brain perfusion scans 1-5 times, respectively. After scanning, the cell number was counted after 10-day continuous culture and cell growth curve of each tube was drawn. Cell cycle was detected by flow cytometry, and cell viability was measured by MTT method.

RESULTS AND CONCLUSION: After 10 days of continuous counting, the number of cells per tube showed no difference (P > 0.05), and cell growth curves were basically coincided. Moreover, there was no significant difference in the cell cycle and cell viability (P > 0.05). Experimental findings show that the CT brain perfusion scan has no effect on the viability of bone marrow mesenchymal stem cells.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Perfusion Imaging, Tomography, Spiral Computed, Tissue Engineering.

Fu Feng-kui, Zhou Hai, Wang Jun. Proliferative activity of bone marrow mesenchymal stem cells cultured in vitro: assessment by a CT brain perfusion scan[J]. Chinese Journal of Tissue Engineering Research, 2016, 20(14): 2027-2032.

Figures/Tables 6

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