Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (45): 7342-7349.doi: 10.3969/j.issn.2095-4344.2015.45.023

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Effects of amniotic fluid stem cell transplantation on immune tolerance and oxidative stress in kidney transplantation

Deng Chun-yang1, Feng Jian-xun2, Zhang Hai-ying2, Chen Ting-fang2, Li Jing3   

  1. 1Health Service Center of the Academy Community, Pudong New Area, Shanghai 201304, China; 2Department of Nephrology, Eastern Branch, Sixth People’ s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201306, China; 3First Affiliated Hospital of Xinjiang Medical University, Urumqi 830000, Xinjiang Uygur Autonomous Region, China
  • Received:2015-09-11 Online:2015-11-05 Published:2015-11-05
  • Contact: Feng Jian-xun, M.D., Chief physician, Department of Nephrology, Eastern Branch, Sixth People’s Hospital Affiliated to Shanghai Jiao Tong University School of Medicine, Shanghai 201306, China
  • About author:Deng Chun-yang, Attending physician, Health Service Center of the Academy Community, Pudong New Area, Shanghai 201304, China
  • Supported by:

    the Natural Science Foundation of Xinjiang Uygur Autonomous Region, No. 2009211A23

Abstract:

BACKGROUND: Stem cells can induce immune tolerance, prolong graft survival time and reduce rejection in organ transplantation, which have become a hot research.
OBJECTIVE: To induce immune tolerance to allogenic kidney transplantation with amniotic fluid stem cells in recipient rats and to explore the mechanism underlying immune tolerance.
METHODS: Amniotic fluid stem cells were isolated from Wistar rats. Two inbred male rat strains, Wistar rats and Sprague-Dawley rats, were selected as donors and recipients of kidney transplantation. The rat models of renal orthotopic transplantation were divided into the following four groups: a sham-operated group (n=10, Sprague-Dawley rats); an isograft group (n=10, Sprague-Dawley to Sprague-Dawley rats); a control group (n=10, Wistar to Sprague-Dawley rats, treated with 1 mL saline); and an experimental group (n=10, Wistar to Sprague-Dawley rats, treated with 1 mL of 3×106/L amniotic fluid stem cells). Serum levels of creatinine, urea 
nitrogen, interleukin-2, interferon-γ, parameters of oxidative stress were detected at 5 days after operation. Flow cytometry was employed to determine the percentage of CD4+ and CD8+ lymphocytes in the peripheral blood. Kidney transplants were observed pathologically.
RESULTS AND CONCLUSION: Compared with the control group, the levels of creatinine, urea nitrogen, interleukin-2, interferon-γ, parameters of oxidative stress and proteinuria were lower in the experimental group (P < 0.05). Percentages of CD4+, CD8+ and CD4+/CD8+ ratio were also significantly lower in the experimental group than the control group. However, the rate of cretinemia clearance in the experimental group was significantly higher than that in the control group (P < 0.05). Furthermore, the degree of kidney injury in the experimental group was significantly lower than that in the control group. Our findings demonstrate that the amniotic fluid stem cell transplantation can induce immune tolerance, extenuate oxidative stress, attenuate pathological damage to the kidney transplant and preserve kidney function from acute rejection in rats undergoing kidney transplantation.
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Stem Cells, Amniotic Fluid, Kidney Transplantation, Immune Tolerance, Tissue Engineering