Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (45): 7331-7336.doi: 10.3969/j.issn.2095-4344.2015.45.021

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Neuroprotective effect of neural stem cells modified by glial-derived neurotrophic factor on cerebral apoplexy

Wu Zhong-hua   

  1. Tongzhou District People’s Hospital, Nantong 226399, Jiangsu Province, China
  • Received:2015-09-26 Online:2015-11-05 Published:2015-11-05
  • Contact: Wu Zhong-hua, Tongzhou District People’s Hospital, Nantong 226399, Jiangsu Province, China
  • About author:Wu Zhong-hua, Master, Attending physician, Tongzhou District People’s Hospital, Nantong 226399, Jiangsu Province, China

Abstract:

BACKGROUND: Glial-derived neurotrophic factor has a specific effect on brain neurons, and is an important neurotrophic factor in the treatment of cerebral apoplexy.
OBJECTIVE: To investigate the neuroprotective effect of glial-derived neurotrophic factor gene modified neural stem cells on rat cerebral apoplexy.
METHODS: The recombinant human plasmid pAdEasy-l-pAdTrackCMV was constructed, and the neural stem cells were isolated and cultured from the cortex of neonatal rats. The neural stem cells were transfected with the recombinant adenovirus of glial-derived neurotrophic factor, and the cell suspension was injected into the right brain ventricle of rats with transient cerebral ischemia (2 hours). Meanwhile, neural stem cell transplantation group and control group were set up.
RESULTS AND CONCLUSION: Compared with the neural stem cell transplantation group, the modified neurological severity score of combined transplantation group was reduced significantly 2 and 3 weeks after reperfusion, and the area of cerebral ischemia injury was also significantly decreased at 7 days after reperfusion (P < 0.05). The number of neural stem cells in the neural stem cell transplantation group was significantly less than that in the combined transplantation group (P < 0.05). The expression of Syn, PSD-95 proteins in the two transplantation groups, especially in the combined transplantation group, was higher than that of the control group (P < 0.05). However, there was no significant difference between the two transplantation groups (P > 0.05). The results show that the neural stem cells modified by glial-derived neurotrophic factor can play a better role in the neuroprotection against cerebral apoplexy in rats, and the effect is better than that of simple neural stem cells.
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Tissue Engineering, Stem Cells, Stroke