Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (45): 7325-7330.doi: 10.3969/j.issn.2095-4344.2015.45.020

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Bone marrow mesenchymal stem cell transplantion for repair of airway injury in chronic obstructive pulmonary disease

Zhang Qi, Li Dan   

  1. Department of Respiration, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Received:2015-09-06 Online:2015-11-05 Published:2015-11-05
  • About author:Zhang Qi, Attending physician, Department of Respiration, Zhengzhou Central Hospital Affiliated to Zhengzhou University, Zhengzhou 450000, Henan Province, China
  • Supported by:

    the Scientific Research Plan of Henan Province, No. 142102310166

Abstract:

BACKGROUND: Mesenchymal stem cells can differentiate into lung parenchymal cells involved in lung injury repair, providing a new approach for the application of mesenchymal stem cells in patients with chronic obstructive pulmonary disease.
OBJECTIVE: To observe the effect of bone marrow mesenchymal stem cell transplantation on the repair of airway injury in rats with chronic obstructive pulmonary disease.
METHODS: Twenty-four female rats were randomized into four groups: bone marrow mesenchymal stem cell transplantation group (cell transplantation group, n=12); bone marrow mesenchymal stem cells group (cell control 
group, n=4); model group (n=4); healthy control group (n=4). Rat models of chronic obstructive pulmonary disease were established in the cell transplantation group and model group using fumigation+lipopolysaccharide method; and at 1 day after modeling, model rats were given 1 mL CM-Dil-labeled bone marrow mesenchymal stem cells and 1 mL PBS via the tail vein in these two groups, respectively. In addition to tracheal injection of normal saline (300 μL) at 1 and 14 days, rats in the cell control and healthy control groups were given 1 mL CM-Dil-labeled bone marrow mesenchymal stem cells and 1 mL PBS via the tail vein, respectively. At 1, 7, 15 and 30 days after cell transplantation, lung tissue and serum markers of all rats were detected.
RESULTS AND CONCLUSION: (1) Hematoxylin-eosin staining showed that emphysema and airway injury was milder in the cell transplantation group than the model group, but severer than the cell control and healthy control groups. (2) The total number of leukocytes and neutrophils in the peripheral blood was higher in the cell transplantation group than the cell control and healthy control groups (P < 0.05); with time, the total number of leukocytes and neutrophils was decreased gradually. (3) Compared with the cell control and healthy control groups, the interleukin-10 level in the peripheral blood was lower and the levels of tumor necrosis factor-α and granulocyte colony-stimulating factor were higher at 1 day after cell transplantation (P < 0.05). With time, in the cell transplantation group, the interleukin-10 level was increased gradually, the level of tumor necrosis factor-α was decreased gradually, and the level of granulocyte colony-stimulating factor was increased first and then decreased, which was highest at 7 days after cell transplantation. (4) Partial CM-Dil-positive cells were positive for CC16. Taken together, bone marrow mesenchymal stem cell transplantation via the tail vein can improve lung injury of rats with chronic obstructive pulmonary disease, and it is involved in the repair of airway injury through differentiation into epithelial cells and immune regulation.

Key words: Bone Marrow, Mesenchymal Stem Cell Transplantation, Pulmonary Disease, Chronic Obstructive, Tissue Engineering