Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (45): 7292-7297.doi: 10.3969/j.issn.2095-4344.2015.45.014

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Ginsenosides-induced bone marrow mesenchymal stem cells promote nerve regeneration in traumatic brain injury

Qin Jun, Chen Jia-kang, Li Xue-dong, Mai Yong-jun, Xiao Zhen-yong   

  1. Liuzhou Workers Hospital, Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545000, Guangxi Zhuang Autonomous Region, China
  • Received:2015-09-29 Online:2015-11-05 Published:2015-11-05
  • Contact: Chen Jia-kang, Chief physician, Liuzhou Workers Hospital, Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545000, Guangxi Zhuang Autonomous Region, China
  • About author:Qin Jun, Attending physician, Liuzhou Workers Hospital, Fourth Affiliated Hospital of Guangxi Medical University, Liuzhou 545000, Guangxi Zhuang Autonomous Region, China

Abstract:

BACKGROUND: Previous studies have shown that bone marrow mesenchymal stem cells in the treatment of neurological diseases have achieved some success, which can promote neurological alterations; however, there is no breakthrough on gene and drug regulation.
OBJECTIVE: To investigate the influence of ginsenosides-induced differentiation of bone marrow mesenchymal stem cells on nerve regeneration after traumatic brain injury.
METHODS: A traumatic brain injury model was built in rats using hydraulic shock method, and then rat models were randomly divided into model group (traumatic brain injury group), bone marrow mesenchymal stem cell group, ginsenosides group (ginsenosides induced differentiation of bone marrow mesenchymal stem cells). At 2 weeks after transplantation, western blot assay was used to detect protein expression levels of nerve growth factor and brain-derived neurotrophic factor, immunohistochemistry assay used to detect the number of BrdU-positive cells. At 1, 3 days and 1, 2 weeks after transplantation, modified neurological severity scores were recorded.
RESULTS AND CONCLUSION: The expression levels of nerve growth factor and brain-derived neurotrophic factor protein were significantly higher in the ginsenosides group than the bone marrow mesenchymal stem cell 
group and model group (P < 0.05). The number of BrdU positive nerve cells was also higher in the ginsenosides group than the bone marrow mesenchymal stem cell group and model group (P < 0.05). At 3 days and 1, 2 weeks after transplantation, the modified neurological severity scores in the ginsenosides group were lower than those in the bone marrow mesenchymal stem cell group and model group (P < 0.05). These findings indicate that ginsenoside-induced bone marrow mesenchymal stem cell transplantation can promote nerve regeneration in rats with traumatic brain injury, which has better outcomes than bone marrow mesenchymal stem cell transplantation alone.
中国组织工程研究杂志出版内容重点:干细胞;骨髓干细胞;造血干细胞;脂肪干细胞;肿瘤干细胞;胚胎干细胞;脐带脐血干细胞;干细胞诱导;干细胞分化;组织工程

Key words: Craniocerebral Trauma, Ginsenosides, Bone Marrow, Mesenchymal Stem Cell Transplantation, Tissue Engineering