Kallikrein 1-transfected bone marrow mesenchymal stem cells: selection of the multiplicity of infection

doi:10.3969/j.issn.2095-4344.2015.45.006

Abstract

Abstract:

BACKGROUND: Kallikrein 1 is an important component of the kallikrein-kinin system. Studies have shown that kallikrein can protect the cardiovascular system by promoting angiogenesis and inhibiting myocardial inflammation, but there is no report on its effect on inducing differentiation of stem cells.
OBJECTIVE: To determine the transfection efficiency of kallikrein 1 adenoviral vector in rat bone mesenchymal stem cells.
METHODS: Using adenovirus as a vector, the target gene kallikrein 1 was transfected into rat bone marrow mesenchymal stem cells. Fluorescence microscopy, MTT method and flow cytometry were used to investigate the effect of transfection and determine the optimal multiplicity of infection.
RESULTS AND CONCLUSION: Adenovirus carrying kallikrein 1 was successfully transfected into rat bone marrow mesenchymal stem cells. Results from flow cytometry showed that the transfection efficiency was associated with the multiplicity of infection. When the multiplicity of infection was 150, the transfection efficiency was 80.8%. MTT results showed that when the multiplicity of infection was 200, the cell growth was inhibited remarkably. These findings indicate that adenovirus-mediated kallikrein 1 can be successfully transfected into rat bone marrow mesenchymal stem cells with the optimal multiplicity of infection=150.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Engineering, Stem Cells, Transfection, Green Fluorescent Proteins

Jia Jia, Jin Li-mei, Zhao Yi, Yan Li, Lu Juan, Wang Qiu-ping, Zhao Jing-miao, Hu Ji-hong. Kallikrein 1-transfected bone marrow mesenchymal stem cells: selection of the multiplicity of infection[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(45): 7249-7253.

Figures/Tables 4

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