Effect of adipose-derived stem cells modified by insulin-like growth factor I gene on TWEAK/Fn14 signaling pathway

doi:10.3969/j.issn.2095-4344.2015.45.001

Abstract

Abstract:

BACKGROUND: Because insulin-like growth factor I has the ability to induce mesenchymal stem cells into chondrocytes, we hypothesized that the chondrogenic differentiation of adipose-derived stem cells can be improved via insulin-like growth factor I transfection.

OBJECTIVE: To investigate the influence of insulin-like growth factor I transfection on chondrogenic potential of adipose-derived stem cells in vitro and tumor necrosis factor-like weak inducer of apoptosis (TWEAK)/fibroblast growth factor-inducible 14 (Fn14) signaling pathway.

METHODS: Recombinant lentivirus plasmid pLVX-IGF-I-IRES-ZsGreenl was constructed and transferred into passage 3 adipose-derived stem cells, and then these stem cells were induced to differentiate into chondrocytes (experimental group 1). Meanwhile, the cells transfected with pLVX-IRES-ZsGreenl were taken as green fluorescent protein/adipose mesenchymal stem cell group (experimental group 2), and those with no transfection acted as control group.

RESULTS AND CONCLUSION: The mRNA expression of TWEAK was reduced in the experimental group 1 as compared with the other two groups, but the mRNA expressions of insulin-like growth factor I, Col2a1 and Sox9 were up-regulated in the experimental group 1. At the same time, the protein expression of matrix metalloproteinase-3 and TWEAK were down-regulated, while the protein expression of Col2a1 was increased in the insulin-like growth factor I-transfected cells in contrast to the cells modified with pLVX-IRES-ZsGreenl or with no transfection. These findings indicate that pLVX-IGF-I-IRES-ZsGreenl transfection of adipose-derived stem cells results in a higher expression of insulin-like growth factor I, and down-regulates the expression of TWEAK mRNA and protein, which improves the differentiation of adipose-derived mesenchymal stem cells into chondrocytes.
中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Chondrocytes, Insulin-Like Growth Factor I, Transfection, Tissue Engineering

Liu Yu-ping, Wang Ming-ming, Liu Tao, Li Ming, Yu Guang-rong. Effect of adipose-derived stem cells modified by insulin-like growth factor I gene on TWEAK/Fn14 signaling pathway[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(45): 7217-7223.

Figures/Tables 7

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