Preparation and performance of three kinds of thermoresponsive acellular carriers

doi:10.3969/j.issn.2095-4344.2015.43.022

Abstract

Abstract:

BACKGROUND: To design and fabricate a novel three-dimensional thermoresponsive polymer cell scaffold is one of the hot topics in the research of polymer science.
OBJECTIVE: To prepare three different kinds of thermoresponsive acellular carriers and to evaluate their performance.
METHODS: The copolymer N-isopropylacrylamide temperature acellular scaffold, macroporous copolymer N-isopropylacrylamide temperature acellular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acellular scaffold were prepared. The specific surface area, thermoresponsive performance, porosity, pore size and biocompatibility of these three groups of scaffolds were determined.
RESULTS AND CONCLUSION: The specific surface area of copolymer N-isopropylacrylamide
thermoresponsive acellular scaffold, macroporous copolymer N-isopropylacrylamide thermoresponsive acellular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive cellular scaffold was respectively 135, 386, 421 m2/g. The lower critical solution temperature was 30, 28.5, 29.5 ℃. The cell toxicity reaction was respectively grade 2, 2, 1. These indicators showed that the three kinds of scaffolds were provided with a temperature-sensitive characteristics and similar lower critical solution temperature. The biocompatibility of macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acellular scaffold was significantly better than the other two scaffolds. The porosity and pore size of macroporous copolymer N-isopropylacrylamide thermoresponsive acellular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acellular scaffold were greater than those of the copolymer N-isopropylacrylamide thermoresponsive acellular scaffold (P < 0.05). These results demonstrate that macroporous copolymer N-isopropylacrylamide thermoresponsive acellular scaffold and macroporous copolymer N-isopropylacrylamide crosslinking aldehyde sodium alginate thermoresponsive acellular scaffold have more obvious pore structure.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Scaffolds, Histocompatibility Testing, Liver, Artificial, Tissue Engineering

An Shuai-xing, Yu Mei-li, Guo Hong-yue, Li Tan, Li Yang. Preparation and performance of three kinds of thermoresponsive acellular carriers[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(43): 7004-7009.

Figures/Tables 3

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