Comparison of in vitro cytotoxicity between calcium alginate hemostatic dressing and three kinds of commonly used clinical hemostatic dressings

doi:10.3969/j.issn.2095-4344.2015.43.021

Abstract

Abstract:

BACKGROUND: Hemostatic dressing can directly contact with the body tissues on the wound surface. The biocompatibility is one of the important indicators of evaluating the advantages and disadvantages of dressing. The hemostatic dressing prepared with calcium alginate as raw material has become a research focus owing to its low cost and good compatibility.

OBJECTIVE: To observe the cytotoxicity of calcium alginate hemostatic dressings and to compare the cytotoxicity between calcium alginate hemostatic dressing and gelatin hemostatic sponge, absorbing cotton, ordinary gauze.

METHEDS: Leaching solution method: the DMEM high glucose culture solution was taken as the leaching medium. The calcium alginate hemostatic dressing, gelatin hemostatic sponge, absorbing cotton and ordinary gauze leaching solution were respectively prepared. Five concentration gradients of 100%, 75%, 50%, 25%, 50% were set. The fibroblast cells of L-929 mouse were cultured for 24 hours with the above material leaching solution. The volume fraction of 10% DMEM high glucose culture medium was taken as control group, and DMEM high glucose culture medium containing 5% DMSO was taken as positive control group to observe the cell proliferation and morphological changes. Direct contact method: The fibroblast cells of L-929 mouse were respectively seeded in calcium alginate hemostatic dressing, gelatin hemostatic sponge, absorbing cotton and ordinary gauze and cultured for 24 hours. The changes in cell morphology were observed.

RESULTS AND CONCLUSION: Leaching solution method: The cytotoxicity of alginate fiber hemostatic dressing, gauze, absorbing cotton leaching solution with different concentration gradients was grade 1, which was in line with GB/T16886/ ISO10993 biological evaluation standard of medical apparatus and instruments. The cytotoxicity of 100%, 75% gelatin hemostatic sponge extract solution was grade 3, causing severe inhibition of cell proliferation. Direct contact method: The cytotoxicity of gauze and alginate fiber hemostatic dressing was grade 1, absorbing cotton was grade 2, gelatin hemostatic sponge was grade 3. These results demonstrate that calcium alginate hemostatic dressing has no cytotoxicity.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Biological Dressings, Gelatin Sponge, Absorbable, Surgical Sponges, Tissue Engineering

Chu Xiao-xia, Wang Bin, Wang Hai-tao, Chen Hao, Cui Fei-yan. Comparison of in vitro cytotoxicity between calcium alginate hemostatic dressing and three kinds of commonly used clinical hemostatic dressings[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(43): 6998-7003.

Figures/Tables 4

References

[1] 孙玉玲,刘杰.医用敷料用海藻纤维研究进展[J].科技信息,2010, 27(32):134-135.

[2] 黄若昆.可注射性骨组织工程载体研究进展[J].中国矫形外科杂志,2007,15(9):681-683.

[3] Jayakumar R,Prabaharan M,Sudheesh Kumar PT,et al. Biomaterials based on chitin and chitosan in wound dressing applications.Biotechnol Adv.2011;29(3):322-337.

[4] Lee KY, Mooney DJ. Alginate: properties and biomedical applications. Progr Polym Sci.2012;37(1):106-126.

[5] 汪涛,赵珺,张震,等.海藻酸钙敷料的细胞毒性体外测试结果分析[J].中国现代普通外科进展,2014,17(9):692-696.

[6] Kasseroller RG,Benner E.A prospective randomised study of algina tedrenched low stretch bandages as an alternative to conventional lymphologic compression bandaging. Support Care Cancer.2010;18(3):343-350.

[7] 秦益民.海藻酸纤维在医用敷料中的应用[J].合成纤维,2004, 32(4): 11-16.

[8] 张林朴,王冠华,连小丽,等.海藻酸钠/壳聚糖复合凝胶的制备与细胞毒性评价[J].中国组织工程研究,2014,18(21):3310-3315.

[9] 贾文英,史弘道.细胞毒性试验评价医用装置生物相容性的研究概况[J].实用美容整形外科杂志,2001,12(5):255-256.

[10] ISO,International Organization for Standardization.Biological evaluation of medical devices-Part 5:Test for in vitro cytotoxicity. International Organization for Standardization, 2009.

[11] 刘茜,兰华林,顾铭,等.贻贝粘蛋白创面修复敷料体外细胞毒性的检测方法[J].中国组织工程研究,2013,17(38):6785-6790.

[12] Gomes ME,Reis RL,Cunha AM,et al.Cytocompatibility and response of osteblastic-like cells to starch-based polymers: Effect of several additives and processing conditions. Biomaterials.2001;22(13):1911-1917.

[13] 奚延雯.组织工程医疗产品安全性评估[J].现代康复,2001,5(6): 13-15.

[14] 国家质量监督检验检疫总局.GB/T16886.5 医疗器械生物学评价第5部分:体外细胞毒性试验,2003.

[15] Royal College of Nursing. The Management of Pressure Ulcers in Primary and Secondary Care: a Clinical Practice, Guideline. London: Royal College of Nursing, UK.2005.

[16] 林晓华,黎志超,俞金龙,等.新型复合生物抗菌敷料的抗菌强度、吸湿能力及细胞毒性[J].中国组织工程研究,2013,17(16): 2905-2912.

[17] Pielesz A,Katarzyna B,Klimczak M.Physico-chemical properties of commercial active alginate dressings.Polirn Med.2008;38(4):3-17.

[18] 杨建德,韦元强,莫海龙,等.复合皮片移植治疗烧伤后功能部位瘢痕20例[J].中国组织工程研究与工临床康复,2007,11(8): 1544-1545．

[19] 李丽,周建平,胡宇驰.细胞浓度和作用时间对细胞毒性检查法影响的探讨[J].中国实验方剂学杂志,2012,18(14):101-104.