Acellular dermal matrix for repair of porcine bile duct defects: to promote vascular and bile duct epithelial regeneration

doi:10.3969/j.issn.2095-4344.2015.43.010

Abstract

Abstract:

BACKGROUND: Acellular dermal matrix is a cell-free natural tissue scaffold similar to human soft tissue, which is easy to shape and has non-toxic side effects. It has been used to repair the urethra and ureter.
OBJECTIVE: To investigate the effect of acellular dermal matrix on the repair of bile duct injury.
METHODS: Thirty Diannan miniature pigs were randomly divided into three groups: in blank group, the bile duct was resected followed by end to end anastomosis; in experimental group, bile duct defect model was made followed by repair with acellular dermal matrix; in control group, bile duct defect model was made followed by repair with expanded polytetrafluoroethylene. At 6 and 24 weeks after repair, bile duct patches and surrounding tissues were taken for immunohistochemical observation and RT-PCR detection.
RESULTS AND CONCLUSION: Compared with the control and blank group, the expression of cytokeratin was higher, but the expression of transforming growth factor β1 was lower in the experimental group. Within 24 weeks after repair, the total mRNA level of transforming growth factor β1 was lower in the experimental group than the other two groups (P < 0.05), but the total mRNA levels of insulin-like growth factor 2 and vascular endothelial growth factor were higher in the experimental group (P < 0.05). These findings indicate that the acellular dermal matrix for repair of bile duct injury can promote angiogenesis and bile duct epithelial regeneration, but not increase the formation of scars.
中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Bile Ducts, Insulin-Like Growth Factor II, Transforming Growth Factor beta1, Tissue Engineering

Chen Gang, Bai Jian-hua, Zhu Xin-feng, Cao Jun, Liu Qi-yu, Zhao Ying-peng, Li Li. Acellular dermal matrix for repair of porcine bile duct defects: to promote vascular and bile duct epithelial regeneration[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(43): 6940-6945.

Figures/Tables 8

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