Application value of echocardiography in establishing rabbit models of diastolic heart failure

doi:10.3969/j.issn.2095-4344.2015.40.005

Abstract

Abstract:

BACKGROUND: Related medical researches have shown that the incidence of simple diastolic heart failure is higher than that of systolic heart failure.

OBJECTIVE: To investigate the application value of echocardiography in the monitoring of rabbit models of simple diastolic heart failure.

METHODS: Sixty healthy male New Zealand rabbits were included in this study. The pressure overload hypertrophy rabbit model was established by the method of abdominal aortic coarctation. Rabbits were randomly divided into surgical group and sham group. All rabbits were subjected to the echocardiography and left heart catheterization, the left ventricular end-diastolic pressure and relaxation time constants were measured to judge the presence of simple diastolic heart failure.

RESULTS AND CONCLUSION: Compared with the sham group, the aortic annular diameter was significantly reduced before surgery and at 1, 4, 8 weeks after surgery in the model group (P < 0.05); the peak of retrograde velocity at atrial contraction was decreased in the model rabbits at 8 weeks after surgery (P < 0.05); the left ventricle dry weight, ratio of left ventricle dry weight/body weight, left ventricular posterior wall thickness, systolic pressure, interventricular septum, left ventricle systolic pressure, and left ventricular end-diastolic pressure were obviously increased at 8 weeks after surgery (P < 0.05). Experimental findings indicate that rabbit models of simple diastolic heart failure are successfully established, and echocardiography can effectively monitor the left ventricular systolic and diastolic functions in cardiac hypertrophy.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Echocardiography, Stress, Heart Failure, Diastolic

CLC Number:

R318

Gong Li-yuan, Huang Wei, Chen Jian-ming, Lin Yang-yuan. Application value of echocardiography in establishing rabbit models of diastolic heart failure[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(40): 6418-6422.

Figures/Tables 2

References

[1] 杜纪兵,陈闽荔,郭玉军,等.左西孟旦在老年舒张性心力衰竭患者中的应用[C].中华医学会第十五次全国心血管病学大会论文汇编.2013.
[2] 齐明旭.血尿酸与代谢综合征对舒张性心力衰竭的交互影响[D].衡阳:南华大学,2013.
[3] 梁健球.关于心肌梗死后心力衰竭患者血浆中miRNA表达及基于ECHO的心肌能量消耗的研究[D].广州,南方医科大学, 2013.
[4] 兰志超,杨生华,郭淑丽.辛伐他汀联合氨氯地平治疗舒张性心力衰竭的疗效[J].中国医药指南,2013,(11):607-608.
[5] 黄飞飞,蒋金法.超声心动图新技术在慢性心力衰竭心脏同步化评估中的应用[J].心血管病学进展,2014,(2):175-178.
[6] 韩彤亮,康维强.超声心动图评价舒张性心力衰竭的适用标准与合理应用[J].中国循环杂志,2013,(2):160-162.
[7] 张潇怡,任菲,江鹏程,等.老年舒张性心力衰竭患者的临床特点及心功能评价方法[J].中国医科大学学报,2013,(2):111-113.
[8] 支晓明,李必龙.米力农联合利尿剂对顽固性心力衰竭患者超声心动图及脑钠肽的影响[J].实用临床医药杂志,2013,(1): 76-80.
[9] 吴彬,孟纯阳,王海滨,等.颈椎不稳对邻近节段椎间盘蛋白多糖及Ⅱ型胶原的影响[J].中国组织工程研究,2013,(30): 5421- 5426.
[10] 解道宇,鞠建宝,于海玲,等.兔适合构建鼻窦炎动物模型(英文)[J].中国组织工程研究,2014,18(18):2830-2835.
[11] Komamura K, Westermann D. Similarities and differences between the pathogenesis and pathophysiology of diastolic and systolic heart failure. Cardiol Res Pract. 2013;2013: 824135.
[12] Pourrier M, Williams S, McAfee D, et al. Crosstalk proposal: the late sodium current is an important player in the development of diastolic heart failure (heart failure with a preserved ejection fraction). J Physiol. 2014;592(Pt 3): 411-414.
[13] Papp Z, Borbély A, Paulus W. Cross talk opposing view: The late sodium current is not an important player in the development of diastolic heart failure (heart failure with a preserved ejection fraction). J Physiol. 2014;592(Pt 3): 415-417.
[14] Åsmund T, Aronsen JM, Frisk M. et al. Compensated cardiomyocyte Ca2+ homeostasis in a rat model of diastolic heart failure. Biophys J. 2013;104(2):603A.
[15] Namsik Yoon, Jeong Cho, Kye Kim.etal. Beneficial effects of an angiotensin?II receptor blocker on structuralatrial reverse?remodeling in a rat model of ischemic heart failure. Exp Ther Med. 2013;5(4):1009-1016.
[16] Dewan S, Allen EJ, Geenen DL, et al. Myofilament dysfunction in a guinea-pig model of diastolic heart failure. Biophys J. 2009;96(3):619a-620a.
[17] Guo HLi, Yu S, Yu C, et al. The role of autophagy in iron-overload cardiomyopathy: a model of diastolic heart failure due to oxidative stress. J Cardiac Fail. 2009;15(6): S42-S43.
[18] Shay DB, Yaron Arbe l. Large animal models for diastolic dysfunction and diastolic heart failure-a review of the literature. Cardiovasc Pathol. 2010;19(3):147-152.
[19] Mathieu M, El Oumeiri B, Touihri K, et al. Ventricular-arterial uncoupling in heart failure with preserved ejection fraction after myocardial infarction in dogs-invasive versus echocardiographic evaluation. BMC Cardiovasc Disord. 2010;10(1):32.
[20] Yin J, Kukucka M, Hoffmann J, et al. Sildenafil preserves lung endothelial function and prevents pulmonary vascular remodeling in a rat model of diastolic heart failure. Circ Heart Fail. 2011;4(2):198-206.
[21] Westermann C, Riad A, Richter U, et al. Enhancement of the endothelial NO synthase attenuates experimentaldiastolic heart failure. Basic Res Cardiol. 2009;104(5):499-509.
[22] Deng AY, Nattel S, Shi YF, et al. Distinct genomic replacements from Lewis correct diastolic dysfunction, attenuate hypertension, and reduce left ventricular hypertrophy in Dahl salt-sensitive rats. J Hypertens. 2008;26(10):1935-1943.
[23] Szardien S, Nef H, Liebetrau C. G-CSF leads to a normalization of diastolic function in a mouse model of diastolic heart failure. Eur Heart J. 2012;33:35-35.
[24] Tsutsui M, Shibata K, Yatera Y. A novel murine model of diastolic heart failure: mice lacking all nitric oxide synthases. J Pharmacol Sci. 2010;112:244.
[25] Deng Alan Y , Stanley N, Shi YF, et al. Distinct genomic replacements from Lewis correct diastolic dysfunction, attenuate hypertension, and reduce left ventricular hypertrophy in Dahl salt-sensitive rats. J Hyperten. 2008; 26(10):1935-1943.
[26] Hiromitsu M, Katsuyuki A, Hiroo K, et al. Salt excess causes left ventricular diastolic dysfunction in rats with metabolic disorder. Hypertension. 2008;52(2):287-294.
[27] Sun YY, Liu GH, Song T, et al. Upregulation of GRP78 and caspase-12 in diastolic failing heart. Acta Biochimica Polonica. 2008;55(3):511-516.
[28] Wojciech P, Krzysztof G, Jan S, et al. Diastolic heart dysfunction, increased pulmonary capillary wedge pressure and impaired exercise tolerance in patients with systemic sclerosis. Kardiologia Polska. 2011;69(3):243-249.
[29] Anstadt Mark P, Subbaraju B, Rebecca J, et al. Ventricular actuation improves systolic and diastolic myocardial function in the small failing heart. Ann Thorac Surg. 2009;88(6): 1982-1988.