CAD model design for three-dimensional printing of tissue-engineered tooth scaffold

doi:10.3969/j.issn.2095-4344.2015.38.023

Abstract

Abstract:

BACKGROUND: There are less reports on how to successfully build the internal spatial configuration of tissue-engineered tooth scaffolds.

OBJECTIVE: To find a way to establish a series of three-dimensional digital modes for tissue-engineered tooth scaffold, such as CAD spatial configuration and Standard Template Library (STL) files.

METHODS: In order to get three-dimensional printing format of STL files, MICRO CT data of DICOM format were input into MIMICS and GEOMAGIC softwares, creating the outline of STL files. Then CATIA V5R17 software was used to create the three-dimensional digital mode of tissue-engineered tooth. Then, the overall model of the internal scaffold was obtained by arraying at the proper coordinates. Various overall scaffold configurations could be built rapidly by varying monomer configuration. The STL files of CAD model of three-dimensional printing tissue-engineered tooth were obtained by assembling the tooth outline mode and the internal scaffold.

RESULTS AND CONCLUSION: The CAD model was constructed successfully, and this model could be directly used for three-dimensional printing rapid prototyping system to produce tissue-engineered tooth scaffolds. These findings indicate that the three-dimensional digital mode based on reverse engineering and positive engineering can be established, which can be used to quickly build a variety of internal spatial configurations of scaffold materials required for tissue-engineered teeth.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Computer-Aided Design

CLC Number:

R318

Zhang Jia-yu, Mi Xue, Liu Yi, He Hui-yu. CAD model design for three-dimensional printing of tissue-engineered tooth scaffold[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(38): 6195-6199.

Figures/Tables 1

References

Barron JA,Wu P,Ladouceur HD,et al.Biological laser printing: a novel technique for creating heterogeneous 3-dimensional cell patterns.Biomed Microdevices.2004;6(2):139-147.
[2] Ikeda E,Morita R,Nakao K,et al.Fully functional bioengineered tooth replacement as an organ replacement therapy.Proc Natl Acad Sci U S A.2009;106(32):13475-13480.
[3] Honda MJ,Ohara T,Sumita Y,et al.Preliminary study of tissue-engineered odontogenesis in the canine jaw. J Oral Maxillofac Surg.2006;64(2):283-289.
[4] Honda MJ,Tsuchiya S,Sumita Y,et al.The sequential seeding of epithelial and mesenchymal cells for tissue-engineered tooth regeneration.Biomaterials.2007; 28(4):680-689.
[5] Ryan GE,PanditA S,APatsidis DP.Porous itanium scaffolds fabricated using a Rapid PrototyPing and Powder metallurgy teehnique.Biomaterials.2008;29(27):3625-3635.
[6] Will J,Meleher R,Treul C,et al.Porous ceramic bones scaffolds for vascularized Bone tissue regeneration.J Mater Sci Mater Med.2008;19(8):2781-2790.
[7] Suwan Prateeb J,Sanngam R,SuwanPreuk W.Fabrication of bioactive hydroxyapatite/bis-GMA based composite via three dimensional printing.J Mater Sci Mater Med. 2008;19(7): 2637-2645.
[8] Ausiello P,Apicella A,Davidson CI.Effect of adhesive layer properties on stress-distribution in composite restoration-a 3D finite element analysis.Dent Mat.2002;18(4):295-303.
[9] Ciocca L,De Crescenzio F,Fantini M,et al.CAD/CAM and rapid prototyped scaffold construction for bone regenerative medicine and surgical transfer of virtual planning: a pilot study.Comput Med Imaging Graph.2009;33(1):58-62.
[10] Sohmura T,Kusumoto N,Otani T,et al.CAD/CAM fabrication and clinical application of surgical template and bone model in oral implant surgery.Clin Oral Implants Res. 2009;20(1): 87-93.
[11] Ouyang HW,Cao T,Zou XH, et al. Mesenchymal stem cell sheets revitalize nonviable dense grafts:implications for repair of large-bone and tendon defects. Transplantation. 2006; 82(2):170-174.
[12] Wu C,Luo Y,Cuniberti G,et al.Three-dimensional printing of hierarchical and tough mesoporous bioactive glass scaffolds with a controllable pore architecture, excellent mechanical strength and mineralization ability.Acta Biomater.2011;7(6): 2644-2650.
[13] Mironov V,Boland T,Trusk T,et al.Organ printing computer-aided jet-based 3D tissue engineering.Trends Biotechnol.2003;21(4):157-161.
[14] Kastrav WE,Palazzolo RD,Rowe CW,et al.Oral dosage forms fabricated by three dimensional printing. J Control Release. 2000;66:1.
[15] Franco J,Hunger P,Launey ME,et al.Direct write assembly of calcium phosphate scaffolds using a water-based hydrogel. Acta Biomater.2010;6(1):218-228.
[16] Stoppato M,Carletti E,Sidarovich V,et al.Influence of scaffold pore size on collagen I development: A new in vitro evaluation perspective.J Bioact Compat Pol.2013;28:16-32.
[17] Sohmura T,Kusumoto N,Otani T,et al.CAD/CAM fabrication and clinical application of surgical template and bone model in oral implant surgery.Clin Oral Implants Res. 2009;20(1): 87-93.
[18] Charlton DC,Peterson MG,Spiller K,et al.Semi-degradable scaffold for articular cartilage replacement.Tissue Eng Part A.2008;14(1):207-213.
[19] Mitsak AG,Kemppainen JM,Harris MT,et al.Effect of polycaprolactone scaffold permeability on bone regeneration in vivo.Tissue Eng Part A.2011;17(13-14):1831-1839.
[20] 张富强,王运赣.快速成形技术在生物医学领域中的应用[M].北京:人民军医出版社,2009.
[21] Hench LL,Thompson I.Twenty-first century challenges for biomaterials.J R Soc Interface.2010;7 Suppl 4:S379391.
[22] Cao H, Kuboyama N: A biodegradable porous composite scaffold of PGA/beta-TCP for bone tissue engineering.Bone. 2010;46:386-395.
[23] Sultana N, Wang M.Fabrication of HA/PHBV composite scaffolds through the emulsion freezing/freeze-drying process and characterisation of the scaffolds.J Mater Sci Mater Med. 2008;19(7):25552561.
[24] Rao RB,Krafcik KL,Morales AM,et al.Microfabricated deposition nozzles for direct-write assembly of three-dimensional periodic structures.Adv Mater 2005; (17):289-282.
[25] Ghosh S,Parker ST,Wang XY,et al.Direct-write assembly of microperiodic silk fibroin scaffolds for tissue engineering applications.Adv Funct Mater.2008;(18):1883-1889.

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