Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (38): 6179-6183.doi: 10.3969/j.issn.2095-4344.2015.38.020

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5-Fluorouracil-loaded polylactic acid nanoparticles have a killing effect on gastric cancer cell lines in vitro

Li Xiao-li1, Niu Min1, Zhang Ming1, Zhang Na-na1, Shi Yao2   

  1. 1Department of Gastroenterology, Xi’an Honghui Hospital, Xi’an 710054, Shaanxi Province, China;
     2Department of Gastroenterology, the First Affiliated Hospital of Medical College of Xi’an Jiaotong University, Xi’an 710061, Shaanxi Province, China
  • Contact: Li Xiao-li, Department of Gastroenterology, Xi’an Honghui Hospital, Xi’an 710054, Shaanxi Province, China
  • About author:Li Xiao-li, Associate chief physician, Department of Gastroenterology, Xi’an Honghui Hospital, Xi’an 710054, Shaanxi Province, China
  • Supported by:

    the Scientific Research Foundation of the First Affiliated Hospital of Medical College of Xi’an Jiaotong University, No. 2014YK.5

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Abstract:

BACKGROUND: 5-Fluorouracil occupies an important position in the treatment of gastric cancer, but its long-term use can easily induce adverse reactions such as myelosuppression and leukopenia. Polylactic acid and its copolymers have a higher biocompatibility, and their decomposer cannot gather in the body.

OBJECTIVE: To investigate the in vitro cytotoxicity mechanism of 5-fluorouracil-loaded polylactic acid nanoparticles on gastric cancer cell lines.
METHODS: Ten mice were selected in this study. 5-fluorouracil-loaded polylactic acid nanoparticles (1×10-7, 1×10-6, 1×10-5, 1×10-4 mol/L) were prepared using ultrasonic emulsification method. Killing effect of polylactic acid nanoparticles on gastric cancer cell lines in vitro were detected. Then, the inhibition rate was calculated at different concentrations.
RESULTS AND CONCLUSIONS: Under the transmission electron microscope, 5-fluorouracil-loaded polylactic acid nanoparticles had good shape and relatively evenly distributed with no adhesions. After drug administration, the drug concentration was 50% at 24 hours and 62.9% at 72 hours. After 48 hours co-culture with single 5-fluorouracil or 5-fluorouracil-loaded polylactic acid nanoparticles, the viability of gastric cancer cells showed a decrease trend with the increase of drug concentrations, and moreover, 5-fluorouracil-loaded polylactic acid nanoparticles had a better cell inhibition ability than the single 5-fluorouracil (P < 0.05). The IC50 value of 5-fluorouracil-loaded polylactic acid nanoparticles was significantly lower than that of 5-fluorouracil (P < 0.05). These findings indicate that polylactic acid nanoparticles as good drug carriers have a strong drug loading capacity and increase drug concentration in the body, but cannot reduce the biological activity of 5-fluorouracil, which provide new ideas for the treatment of gastric cancer.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Tissue Engineering, Biocompatible Materials, Stomach Neoplasms

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