Bone marrow mesenchymal stem cells/collagen/chitosan combined with bone transport for tibial defect repair: study protocol for a randomized controlled trial

doi:10.3969/j.issn.2095-4344.2015.32.027

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells play an osteogenic role under the assistance of scaffold materials. The scaffold cannot only deliver the cells to the bone defect area, but also act as a new bone growth framework. Collagen-chitosan composite is one of ideal scaffold materials in bone tissue engineering, which has osteoinductive ability and better osteogenic ability than conventional scaffolds. Bone transport technology has been widely used in the clinical repair of long bone defects, but it has some deficiencies, such as slow osteogenesis, long time for external fixation and nonunion. How to further accelerate bone formation and reduce complications has become the current problem to be solved. Here, it is hypothesized that bone marrow mesenchymal stem cells/ collagen/chitosan composite scaffold can increase the therapeutic effect of bone transport in the repair of tibial bone defects.
METHODS/DESIGN: This study is a randomized controlled animal experiment, including in vitro and in vivo tests. In vitro test: Bone marrow mesenchymal stem cells are isolated from the bone marrow of New Zealand rabbits aged 1-2 months, and passaged to the third generation. Then, cell suspension is added onto the collagen-chitosan scaffold to construct the bone marrow mesenchymal stem cells/collagen/chitosan composite scaffold. In vivo test: Twenty-four New Zealand rabbits at 3-4 months are selected and randomly assigned to receive bone transport, scaffold implantation, bone transport+scaffold implantation, respectively. The primary outcome measures are the growth of implant materials and bone defect interface, X-ray detection of bone defect repair, hematoxylin-eosin staining and scanning electron microscope observation of bone formation in the bone defect region, immunohistochemical detection of type I collage expression in the osteogenic region, scanning electron microscope observation of interface bonding between implant materials and host bone, ultrastructure and bone formation.
DISCUSSION: The results from this animal experiment will help to determine the feasibility of bone marrow mesenchymal stem cells/collagen/chitosan composite scaffold to accelerate bone repair during bone defect repair using bone transport technology.

Key words: Stem Cells, Mesenchymal Stem Cells, Tissue Engineering, Tissue Scaffolds, Animals, Biocompatible Materials, Tissue Scaffolds, Collagen, Chitosan, Tibia, External Fixators, Bone Regeneration

CLC Number:

R394.2

Piao Cheng-zhe, Liu Jun, Liu Xin, Ma Yong, Cai Zhen-cun, Shi Dan. Bone marrow mesenchymal stem cells/collagen/chitosan combined with bone transport for tibial defect repair: study protocol for a randomized controlled trial [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5231-5235.

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