In vitro labeled neural stem cells of fetal rats: MRI observation

doi:10.3969/j.issn.2095-4344.2015.32.026

Abstract

Abstract:

BACKGROUND: It is necessary to dynamically monitor the survival, recognition and migration of neural stem cells after implantation.
OBJECTIVE: To in vitro label fetal rat neural stem cells using MRI technology so as to provide applied evidence of neural stem cells in nervous system repair.
METHODS: Fetal rat neural stem cells were isolated, cultured and labeled followed by identification and cell viability detection. A rat model of cerebral ischemia-reperfusion injury was established. Fetal neural stem cells labeled by superparamagnetic iron oxide particles in vitro were transplanted into the left brain of model rats, and unlabeled fetal rat neural stem cells transplanted into the right brain. Prussian blue staining was used to observe the colonization and migration of implanted neural stem cells. MRI tracing was employed to monitor the signal changes of neural stem cells dynamically after in vivo transplantation.
RESULTS AND CONCLUSION: Over 95% fetal rat neural stem cells were labeled successfully by superparamagnetic iron oxide particles, and under electron microscope, there were iron particles in labeled neural stem cells, which were concentrated in the lysosome and endosome. MRI results showed that the labeled neural stem cells had a changing trend of low signals. No difference was found in the cell viability between labeled and unlabeled cells, but T2WI and T2*WI signals were reduced in labeled neural stem cells. These findings confirm that superparamagnetic iron oxide-labeled fetal rat neural stem cells can highly express, and MRI tracing can be used for in vivo monitoring of neural stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Magnetite Nanoparticles, Neural Stem Cells, Magnetic Resonance Imaging

CLC Number:

R394.2

Zheng Zhao-feng, Wang Rong-fang, Wang Qi. In vitro labeled neural stem cells of fetal rats: MRI observation[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5225-5230.

Figures/Tables 4

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