Bone marrow mesenchymal stem cells combined with theaflavin for steroid-induced avascular necrosis of femoral head

doi:10.3969/j.issn.2095-4344.2015.32.023

Abstract

Abstract:

BACKGROUND: Pathological mechanism of femoral head necrosis is not fully known, and high-dose corticosteroids are prone to induce femoral head necrosis.

OBJECTIVE: To investigate the clinical effect of bone marrow mesenchymal stem cells combined with theaflavin on steroid-induced avascular necrosis of femoral head in rats.

METHODS: Rat bone marrow mesenchymal stem cells were isolated and cultured in vitro and compounded onto gelatin sponge, and then, divided into four groups: model group, decompression group, cell transplantation group and combined group. Models of steroid-induced avascular necrosis of femoral head were established using liquid nitrogen method. In the combined group, gelatin sponge carrying bone marrow mesenchymal stem cells was transplanted and 250 mg theaflavin was given daily after modeling.

RESULTS AND CONCLUSION: At 4 weeks after modeling, the femoral head samples in the four groups were round with exfoliation of articular cartilage that was pale in color. Then, the exfoliation of articular cartilage was aggravated in the model group, and some specimens appeared to collapse; the femoral head specimens in the combined group were round and pale. Until the 8^th week after modeling, bone necrosis was increased in the model group; osteoblasts were found in the decompression group with fibrous callus formation; a few of empty bone lacunae appeared with irregular medullary cavity in the cell transplantation group; a large amount of new bone tissues formed in the combined group, and intramedullary adiocytes were in regular array. At 4 and 8 weeks after modeling, the number of empty bone lacunae was significantly lower in the combined group than the other groups (P < 0.05). These findings indicate that bone marrow mesenchymal stem cells combined with theaflavin is an ideal therapy for steroid-induced avascular necrosis of femoral head.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Engineering, Stem Cells, Femur Head Necrosis, Glucocorticoids

CLC Number:

R394.2

Zhao Chuan-yong, Ding Yan-fang, Zhang Wen-zhi, Yao Yu-long. Bone marrow mesenchymal stem cells combined with theaflavin for steroid-induced avascular necrosis of femoral head[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5210-5214.

Figures/Tables 3

References

[1] 曲文娟,马海乐.茶黄素形成机理、制备与分析方法的研究进展[J].食品工业科技,2006,27(6):197-201.
[2] 李府,尚希福,谢涛.茶黄素对鼠骨髓基质干细胞向脂肪细胞诱导分化的影响[J].中国组织工程研究与临床康复,2008,12(16): 3061-3064.
[3] 王江泳,王保芝,刘长安.髓芯减压和骨髓基质细胞移植对家鼠股骨头缺血性坏死的治疗作用[J].中国临床解剖学杂志,2000, 25(2):194-197.
[4] 王江泳,王保芝,崔慧先,等.改良液氮冷冻法制备家鼠股骨头坏死模型的形态学研究[J].河北医科大学学报,2008,1(1):5-7.
[5] 何齐芳,尚希福.茶黄素抑制激素性股骨头坏死的实验研究[J].安徽医科大学学报,2007,42(4):415-419.
[6] 白志刚,刘万林,苏秀兰.细胞因子治疗非创伤性股骨头缺血坏死的研究进展[J].中国矫形外科杂志,2006,14(3):213-215.
[7] 刘兆杰,胡永成.外源性生长因子修饰脂肪组织来源干细胞成骨与成软骨表达的研究进展[J].中国矫形外科杂志,2013,21(3): 250-255.
[8] 白志刚,刘万林,苏秀兰,等.非创伤性股骨头坏死血管内皮生长因子mRNA与骨形成蛋白2表达的实验研究[J].中华实验外科杂志, 2008,25(11):1528.
[9] 白志刚,刘万林,苏秀兰,等.VEGF mRNA与BMP-2在非创伤性股骨头坏死中表达的研究[J].中国矫形外科杂志,2009,17(3):224- 227.
[10] Choi SA, Hwang SK, Wang KC, et al. Therapeutic efficacy and safety of TRAIL-producing human adipose tissue-derived mesenchymal stem cells against experimental brainstem glioma. Neurol Oncol. 2011;13(1):61-69.
[11] Kim NY, Choi YB, Kang CI, et al. An hydrophobically modified arginine peptide vector system effectively delivers DNA into human mesenchymal stem cells and maintains transgene expression with differentiation. J Gene Med. 2010;12(9): 779-789.
[12] Shibatani M, Fujioka M, Arai Y, et al. Degree of corticosteroid treatment within the first 2 months of renal transplantation has a strong influence on the incidence of osteonecrosis of the femoral head. Acta Orthop. 2008;5:631-636.
[13] Chen C, Yang S, Feng Y, et al. Impairment of two types of circulating endothelial progenitor cells in patients with glucocorticoid-induced avascular osteonecrosis of the femoral head. Joint Bone Spine. 2013;1:70-76.
[14] Tingart M, Beckmann J, Opolka A, et al. Analysis of bone matrix composition and trabecular microarchitecture of the femoral metaphysis in patients with osteonecrosis of the femoral head. J Orthop Res. 2009;9:1175-1181.
[15] Chen J, Shi ZD, Ji X, et al. Enhanced osteogenesis of human mesenchymal stem cells by periodic heat shock in self-assembling peptide hydrogel. Tissue Eng Part A. 2013; 5-6:716-728.
[16] 康武林,袁普卫.股骨头坏死与骨髓间充质干细胞移植研究进展[J].中国矫形外科杂志,2013,10(19):1954-1957.
[17] 何伟,陈镇秋,张庆文,等.补肾活血中药治疗股骨头坏死临床研究[J].新中医,2012,44(4):50-51.
[18] 袁普卫,殷继超,朱超,等.骨复生对小鼠镇痛作用的实验研究[J].时珍国医国药,2011,22(1):158-159.
[19] 魏伟,吴希美,李元建.药理实验方法学[M].北京:人民卫生出版社, 2010.
[20] 邵阳,赵晓艳,马勇.激素性股骨头坏死发病机制的研究进展[J].中国中医骨伤科杂志,2011,20(8):88-90.
[21] 李春峰,孙志涛,周正新.不同剂量骨蚀宁胶囊对鼠激素性股骨头坏死微循环的影响[J].中国组织工程研究,2013,17(20):3723- 3729.
[22] Yuan PW, Zhou HZ, He XJ. Experimental study on Gufusheng in Treatment of Steroid-Induced Ischemic Necrosis of Femoral Head in Rabbits. J Tradit Chin Med. 2005;25(4): 300-303.[23] 袁普卫,殷继超,贺西京,等.中药复方骨复生对激素性股骨头缺血性坏死家鼠TNF-α的影响[J].中国骨伤,2004,17(11):662-664.
[24] 李鹏.创伤性股骨头坏死56例的临床分析[J].齐齐哈尔医学院学报,2011,(9):114-115.
[25] 于俊奇.54例创伤性股骨头坏死的临床治疗分析[J].中国保健营养(下旬刊),2012,(11):158-159.
[26] 杨顺云.创伤性股骨头坏死23例的临床治疗体会[J].中国社区医师(医学专业),2011,(5):169-170.
[27] 李明,于立川,张雨,等.创伤性股骨头坏死38例临床治疗体会[J].中外健康文摘,2012,(38):124-125.
[28] 时秋英,刘文华,于晓丽.人工全髋关节置换术治疗晚期股骨头缺血性坏死的护理体会[J].中医正骨,2011,(7):100-102.
[29] 李永奖,蔡春元,杨国敬,等.后外侧微创小切口与传统全髓关节置换术的比较[J].临床骨科杂志,2010,13(2):153-157.
[30] 黄晓文,程力,何天达,等.前外侧小切口与常规后外侧切口全髓关节置换的疗效对比[J].中国组织工程研究,2012,16(9):1538- 1542.
[31] 余敏,王万春,李雨佳.前外侧与后外侧小切口人路行初次全髓置换手术效果比较[J].临床骨科杂志,2012,12(4):445-447.
[32] 崔巍,曲建波,杨方军,等.人工全髓关节置换术后关节脱位与手术人路的临床分析[J].黑龙江医学,2012,33(7):509-510.
[33] 全覃辉,吴良绍,黄兆刚,等.髋关节置换术在股骨头缺血坏死中的治疗作用[J].海南医学院学报,2011,17(6):794.
[34] 梁博伟,潘新元,赵劲民,等.沉默膜联蛋白A1基因影响鼠骨髓间充质干细胞的增殖及成骨分化能力[J].中国组织工程研究与临床康复,2012,16(19):3421-3426.
[35] 张锐东,张澜,毛洪刚,等.激素性股骨头坏死模型中凋亡相关因子的表达[J].中国组织工程研究与临床康复,2013,17(7):1189- 1195.
[36] 暴淑英,赵庆国,毕黎琦.激素性股骨头坏死早期细胞凋亡相关基因表达及阿仑磷酸钠的干预[J].中国组织工程研究与临床康复, 2008,12(46):9095-9099.
[37] 程少华,催超,常巍,等.激素性股骨头缺血性坏死细胞凋亡的实验研究[J].中华实用与治疗杂志,2010,24(4):368.
[38] 胡峰,赵劲民,李晓峰,等.早期激素性股骨头缺血性坏死模型的建立及半胱天冬酶3活性测定[J].中国组织工程研究与临床康复, 2012,14(20):3701-3704.
[39] Li H, Qian W, Weng X, et al. Glucocorticoid receptor and sequential P53 activation by dexamethasone mediatesapoptosis and cell cycle arrest of osteoblastic MC3T3-E1 cells. PLoS One. 2012;7(6):e37030.
[40] Végran F, Boidot R, Solary E, et al. A short caspase-3 isoform inhibits chemotherapy-induced apoptosis by blocking apoptosome assembly. PLoS One. 2011;6(12):e29058.