Wnt signaling promotes the differentiation of adipose mesenchymal stem cells into type II alveolar epithelial cells

doi:10.3969/j.issn.2095-4344.2015.32.012

Abstract

Abstract:

BACKGROUND: Ability of adipose mesenchymal stem cells differentiating into type II alveolar epithelial cells and the regulating mechanism have not been fully elucidated.
OBJECTIVE: To study the ability of adipose mesenchymal stem cells differentiating into type II alveolar epithelial cells in vitro and the function of Wnt pathway in the regulation of differentiation.
METHODS: Adipose mesenchymal stem cells were obtained from fat tissue of rats and identified by flow cytometry. The adipose mesenchymal stem cells were divided into control group, small airway growth medium (SAGM) group and Wnt3a group. Control group was treated with normal DMEM medium; SAGM and Wnt3a groups were both treated with small airway growth medium, and additionally, the Wnt3a group was treated with Wnt3a, a Wnt signaling pathway agonist. After 10 days, quantitative RT-PCR and immunofluorescence detection were used to test the expression of surfactant proteins B, C, D, type II alveolar epithelial markers. Phosphorylated β-catenin and GSK-3β were detected using western blot after 5 and 10 days of induction.
RESULTS AND CONCLUSION: Adipose mesenchymal stem cells with high purity could be successfully isolated from the adipose tissue of rats, and expressed CD44 and CD29, but not CD11b and CD45. After SAGM treatment, protein and mRNA expressions of surfactant proteins B, C, D were all increased in adipose mesenchymal stem cells (P < 0.01), indicating the ability of adipose mesenchymal stem cells to be induced into type II epithelial cells. Surfactant proteins B, C, D expressions at protein and mRNA levels were significantly higher in the Wnt3a group than the SAGM group (P < 0.01). During the induction progress, the expression of phosphorylated β-catenin gradually increased, but GSK-3β expression gradually decreased in the Wnt3a group (P < 0.01). These findings indicate that Wnt signaling pathways are involved in differentiation of adipose mesenchymal stem cells into type II alveolar epithelial cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Stem Cells, Tissue Engineering, Mesenchymal Stem Cells, Pulmonary Alveoli

CLC Number:

R394.2

Shi Li, Jie Jing, Wang Fang, Zhao Dan, Zhang Xiu-fang, Peng Li-ping. Wnt signaling promotes the differentiation of adipose mesenchymal stem cells into type II alveolar epithelial cells[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5148-5154.

Figures/Tables 4

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