MHC antigen expression on the surface of bone marrow stromal stem cells after directional induction in vitro

doi:10.3969/j.issn.2095-4344.2015.32.005

Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (32): 5112-5117.doi: 10.3969/j.issn.2095-4344.2015.32.005

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MHC antigen expression on the surface of bone marrow stromal stem cells after directional induction in vitro

Tong Yan-xiang¹, Feng Wei¹, Suyilaqimuge², Lv Hui-cheng¹, Wu Yi-min¹, Jiang Dian-ming³

1Department of Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China;
2Clinical Research Center, Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China;
3Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, China

Online:2015-08-06 Published:2015-08-06
Contact: Wu Yi-min, M.D., Master’s supervisor, Chief physician, Professor, Department of Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China Corresponding author: Jiang Dian-ming, Doctoral supervisor, Professor, Department of Orthopedics, First Affiliated Hospital of Chongqing Medical University, Chongqing 400016, Chin
About author:Tong Yan-xiang, Studying for doctorate, Department of Orthopedics, Second Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010030, Inner Mongolia Autonomous Region, China
Supported by:
the Million Engineering Project for the Science and Technology of Inner Mongolia Medical University, No. NY2011BW007

Abstract

Abstract:

BACKGROUND: Allogeneic bone marrow stromal stem cell transplantation for treatment of bone diseases is a hot topic. To seek effective methods for prevention of post-transplantation immune rejection is urgent to be solved.
OBJECTIVE: To explore the expression of MHC antigen on the surface of bone marrow stromal stem cells after osteogenic induction in vitro.
METHODS: Bone marrow samples were extracted from rabbits to in vitro isolate and culture bone marrow stromal stem cells. Then, the cells were cultured in IMDM medium containing bone morphogenetic protein-2. Flow cytometry was used to analyze the expression of MHC antigen on the osteoblasts differentiated from bone marrow stromal stem cells.
RESULTS AND CONCLUSION: There was highly expressed MHC I antigen but no MHCII antigen on the osteoblasts differentiated from bone marrow stromal stem cells. After osteogenic induction, no immune rejection was found. These findings indicate that allogeneic or xenogeneic bone marrow stromal cell transplantation can be used in the treatment of bone defects.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells, Cell Differentiation, Osteoblasts, Histocompatibility Antigens

CLC Number:

R394.2

Tong Yan-xiang, Feng Wei, Suyilaqimuge, Lv Hui-cheng, Wu Yi-min, Jiang Dian-ming. MHC antigen expression on the surface of bone marrow stromal stem cells after directional induction in vitro [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5112-5117.

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References

[1] Id Boufker H, Lagneaux L, Najar M, et al. The Src inhibitor dasatinib accelerates the differentiation of human bone marrow-derived mesenchymal stromal cells into osteoblasts. BMC Cancer. 2010;10:298.
[2] Griffin MD, Ryan AE, Alagesan S, et al. Anti-donor immune responses elicited by allogeneic mesenchymal stem cells: what have we learned so far. Immunol Cell Biol. 2013;91(1): 40-51.
[3] Stagg J, Galipeau J. Immune plasticity of bone marrow-derived mesenchymal stromal cells. Handb Exp Pharmacol. 2007;(180):45-66.
[4] Stagg J. Immune regulation by mesenchymal stem cells: two sides to the coin. Tissue Antigens. 2007;69(1):1-9.
[5] Griffin MD, Ritter T, Mahon BP. Immunological aspects of allogeneic mesenchymal stem cell therapies. Hum Gene Ther. 2010;21(12):1641-1655.
[6] Jorgensen C, Djouad F, Apparailly F, et al. Engineering mesenchymal stem cells for immunotherapy. Gene Ther. 2003;10(10):928-931.
[7] Kalbasi Anaraki P, Patecki M, Larmann J, et al. Urokinase receptor mediates osteogenic differentiation of mesenchymal stem cells and vascular calcification via the complement C5a receptor. Stem Cells Dev. 2014;23(4):352-362.
[8] 张晨,王坤正,强辉,等.兔骨髓基质干细胞的体外培养及生物学活性研究[J].西安交通大学学报,2010,31(2):138-142.
[9] Mödder UI, Khosla S. Skeletal stem/osteoprogenitor cells: current concepts, alternate hypotheses, and relationship to the bone remodeling compartment. J Cell Biochem. 2008; 103(2):393-400.
[10] Tan SL, Ahmad TS, Selvaratnam L, et al. Isolation, characterization and the multi-lineage differentiation potential of rabbit bone marrow-derived mesenchymal stem cells. J Anat. 2013;222(4):437-450.
[11] Sanchez-Ramos J, Song S, Cardozo-Pelaez F, et al. Adult bone marrow stromal cells differentiate into neural cells in vitro. Exp Neurol. 2000;164(2):247-256.
[12] 孙健,余优成,顾章愉,等. BMP-2对大鼠骨髓间充质干细胞成骨作用的影响[J].上海口腔医学,2011,20(4): 352-357.
[13] Liu H, Peng H, Wu Y, et al. The promotion of bone regeneration by nanofibrous hydroxyapatite/chitosan scaffolds by effects on integrin-BMP/Smad signaling pathway in BMSCs. Biomaterials. 2013;34(18):4404-4417.
[14] Fang H, Yang X, Chen A, et al. Effect of rhBMP-2 and osteogenic revulsants on proliferation and differentiation of bone marrow stromal cells in rats. J Huazhong Univ Sci Technolog Med Sci. 2007;27(5):561-563.
[15] Tang KC, Trzaska KA, Smirnov SV, et al. Down-regulation of MHC II in mesenchymal stem cells at high IFN-gamma can be partly explained by cytoplasmic retention of CIITA J Immunol. 2008;180(3):1826-1833.
[16] Bartholomew A, Stugeon C, Nelson M, et al. Allogeneic mesenchymal stem cells, hane significant immunosuppressive activity. Exp Hematol. 1999;27:123-139.
[17] Tse WT, Pendleton JD, Beyer WM, et al. Suppression of allogeneic T-cell proliferation by human marrow stromal cells: implications in transplantation. Transplantation. 2003;75(3): 389-397.
[18] Ankrum JA, Ong JF, Karp JM. Mesenchymal stem cells: immune evasive, not immune privileged. Nat Biotechnol. 2014;32(3):252-260.

MHC antigen expression on the surface of bone marrow stromal stem cells after directional induction in vitro

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