Osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells under directional induction

doi:10.3969/j.issn.2095-4344.2015.32.004

Abstract

Abstract:

BACKGROUND: Bone marrow mesenchymal stem cells are non-hematopoietic stem cells from the bone marrow and can differentiate into chondrocytes, osteocytes and adipocytes under different induction conditions, which are the most promising seed cells for tissue engineering.
OBJECTIVE: To evaluate the osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells under directional induction.
METHODS: Bone marrow samples from Japanese rabbits were extracted aseptically to isolate and purify bone marrow mesenchymal stem cells using density gradient centrifugation and cell adherent method. Then, the cells were cultured in osteogenic and adipogenic induction media as experimental group. Another cells cultured in DMEM medium acted as control group. Sudan IV staining, Von Kossa staining and alkaline phosphatase activity detection were performed at different time after culture to compare the osteogenic and adipogenic rates.
RESULTS AND CONCLUSION: After 7 days of adipogenic induction, small lipid droplets were visible and the cells arranged disorderly; at 21 days of induction, see lipid droplets with high refractivity were seen in the cytoplasm. Sudan IV staining showed that there were a large amount of red lipid droplets in the cloning center of bone marrow mesenchymal stem cells, but only 5% bone marrow mesenchymal stem cells differentiated into
adipocytes in the control group. At 7 days of osteogenic induction, induced cells were confluent in a flagstone shape, and there were many nodules; at 14 days of culture, the center of brown mineralized nodules were visible; at 21 days of culture, small pieces of mineralized nodules formed. The osteogenic and adipogenic rates were 40% and 20% in the experimental group, respectively, which were higher than those in the control group (5%, 5%). These findings indicate that under certain conditions, bone marrow mesenchymal stem cells can partially differentiate into fat cells part, and another part differentiate into osteocytes, suggesting there is a certain link between them, that is, more adipocytes and fewer osteoblasts, or vice versa.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Bone Marrow, Mesenchymal Stem Cells;, Cell Differentiation

CLC Number:

R394.2

Zhao Qin-peng. Osteogenic and adipogenic differentiation of bone marrow mesenchymal stem cells under directional induction [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(32): 5103-5107.

Figures/Tables 1

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