Preparation and self-assembly process of fibrillar collagen sponges

doi:10.3969/j.issn.2095-4344.2015.30.013

Abstract

Abstract:

BACKGROUND: An ideal scaffold material needs appropriate degradation rate and certain mechanical properties, but the traditional collagen sponge scaffold has rapid degradation velocity and low mechanical strength, which is easy to collapse and difficult to maintain its natural form. Traditional cross-linking methods also have the problems of cytotoxicity or collagen denaturation, severely limiting the application of collagen.
OBJECTIVE: To design a new cross-linking method for collagen and to optimize the self-assembly process so as to develop a collagen sponge scaffold with good mechanical properties and resistance to degradation.
METHODS: Collagens were modified by self-assembly technology to prepare collagen fibrils which were then freeze-dried into fibrillar collagen sponges. Meanwhile, we optimized the conditions of self-assembly by using orthogonal experiment based on univariate analysis of the effect of initial collagen mass concentration, final phosphate concentration and pH value on the conversion yield of collagen self-assembly.
RESULTS AND CONCLUSION: We optimized the conditions of self-assembly revealed that the optimum
conditions to prepare collagen fibrils were determined as pH=8.0, initial collagen concentration=2 mg/mL, and final concentration of phosphate=15 mmol/L. The results of scanning electron microscope showed that fibrillar collagen sponges were characterized by refined porous structure which was connected by collagen fibrils. In addition, the fibrillar collagen sponges showed better equilibrium-swelling ratio, water retaining property and mechanical strength compared with unmodified collagen spondages (P < 0.05), to solve the problems in rapid degradation.

中国组织工程研究杂志出版内容重点：生物材料；骨生物材料; 口腔生物材料; 纳米材料; 缓释材料; 材料相容性；组织工程

Key words: Collagen, Cross-Linking Reagents

CLC Number:

R318

Zhao Ying, Lu Jin-ting, Deng Chao, Ren Wei-ye, Chen Jing-hua. Preparation and self-assembly process of fibrillar collagen sponges[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(30): 4820-4826.

Figures/Tables 6

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