Chinese Journal of Tissue Engineering Research ›› 2015, Vol. 19 ›› Issue (28): 4520-4525.doi: 10.3969/j.issn.2095-4344.2015.28.017

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Allogeneic adipose-derived stem cells combined with demineralized bone matrix for repair of ulna defects: CT scan and histological detection

Yang Han, Kang Jian-ping, Ding Yu-ming, Wang Song   

  1. First Affiliated Hospital of Sichuan Medical University, Luzhou 646000, Sichuan Province, China
  • Online:2015-07-02 Published:2015-07-02
  • Contact: Wang Song, First Affiliated Hospital of Sichuan Medical University, Luzhou 646000, Sichuan Province, China
  • About author:Yang Han, Master, Physician, First Affiliated Hospital of Sichuan Medical University, Luzhou 646000, Sichuan Province, China
  • Supported by:

    the Natural Science Foundation of Sichuan Medical University, No. 2013ZRQN071

Abstract:

BACKGROUND: Adipose-derived stem cells have a wide variety of sources and strong proliferation ability, which are easy to access and simple to culture. Therefore, adipose-derived stem cells that are secondary to bone marrow mesenchymal stem cells are expected to become the most promising seed cells for bone tissue engineering.

OBJECTIVE: To explore the feasibility of rabbit adipose-derived stem cells with demineralized bone matrix to repair ulna defects.
METHODS: Ulna defect model was made in rabbits. Demineralized bone matrix was implanted into the right defect region as control group. After osteogenic induction, rabbit adipose-derived stem cells/demineralized bone matrix composite was implanted into the left defect region as experimental group. At 12 weeks after implantation, defect tissues were taken for CT scanning and histological detection.
RESULTS AND CONCLUSION: CT results showed that there was unclear boundary between the broken ends of fractured bone and the composite material in the experimental group, and parallel calluses out of the broken end could be seen. In the control group, the broken end was clearly seen and no callus occurred continuously. Fibers were connected at the defect site, and no new bone occurred. Histological findings showed that typical regenerated bone tissues were seen in the experimental group with osteocytes, bone lacunae and bone trabeculae; there were more osteocytes and bone lacunae, but bone trabecula was only seen in a part of bone defects; a few of collagens interlarded the regenerated bone tissues. In the control group, the residual of demineralized bone matrix was seen as well as some collagenous fibers, and periosteal bone formed a little, but no large amount of regenerated osteoid tissues were found. These findings indicate that under osteogenic induction, rabbit adipose-derived stem cells combined with demineralized bone matrix are feasible to repair ulna defects.

Key words: Stem Cells, Adipose Tissue, Tissue Scaffolds, Ulna

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