Effect of suberoylanilide hydroxamic acid combined with hypertonic saline on the hemodynamics of hemorrhagic shock rats

doi:10.3969/j.issn.2095-4344.2015.27.025

Abstract

Abstract:

BACKGROUND: Studies have shown that suberoylanilide hydroxamic acid (SAHA) has protective effects in some vital organs in animals after hemorrhagic shock, and 7.5% hypertonic saline (HS) exerts significant effects on stabilizing the hemodynamics of hemorrhagic shock animals.
OBJECTIVE: To evaluate the effect of SAHA combined with HS on the hemodynamics of hemorrhagic shock rats.
METHODS: Fifty rats were randomly and equally divided into five groups: sham, shock non-resuscitation, SAHA, 7.5% HS, and 7.5% HS + SAHA. Each group contained 10 rats. Except the sham group, rats in the remaining four groups were applied to establish hemorrhagic shock models. In the sham group, rats were given anesthesia catheter, not bleeding; in the shock non-resuscitation group, the bleeding was found, but rats were not resuscitated and were killed after 60 minutes of observations; in the other three groups, rats were respectively resuscitated at 60 minutes after bleeding, through intravenous administration of SAHA within 5 minutes, 7.5% HS and SAHA + 7.5% HS within 20 minutes. Heart rate, mean arterial pressure and left ventricular systolic pressure were monitored through the femoral artery and the right common carotid artery catheter in each group.
RESULTS AND CONCLUSION: At 3 hours after resuscitation, the heart rate was the highest in the 7.5% HS + SAHA group, compared with the SAHA and 7.5% HS groups (P < 0.05). After resuscitation, the mean arterial pressure and left ventricular systolic pressure were increased, with long-lasting effect and less fluctuation (P <
0.05). Experimental results show that 7.5% HS combined with SAHA has a superiority than traditional HS and simple drugs for resuscitation after hemorrhagic shock.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

Key words: Tissue Engineering, Shock, Saline Solution, Hypertonic, Heart Rate

CLC Number:

R318

Liu Jie, Geng Zhi-long . Effect of suberoylanilide hydroxamic acid combined with hypertonic saline on the hemodynamics of hemorrhagic shock rats [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(27): 4400-4404.

Figures/Tables 2

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