Effect of enamel matrix derivatives on the differentiation and proliferation of human periodontal ligament stem cells

doi:10.3969/j.issn.2095-4344.2015.23.019

Abstract

Abstract:

BACKGROUND: The enamel matrix derivative has been used in the clinical treatment of severe periodontitis; however, the mechanism(s) by which enamel matrix derivative promotes periodontal regeneration is still obscure.
OBJECTIVE: To explore the effects of enamel matrix derivatives on the differentiation and proliferation of periodontal ligament stem cells.
METHODS: Periodontal ligament stem cells were isolated and identified from human teeth. Cloning forming efficiency, surface antigen expression and pluripotency were detected and identified. Enamel matrix derivatives with different concentrations (20, 50, 100 mg/L) were used to culture periodontal ligament stem cells for 2 and 4 weeks. Collagen synthesis and mineralized nodule formation were detected using Trichrom staining and Von Kosa’s staining, respectively; real-time RT-PCR was employed to detect expressions of collagen type I, osteocalcin, and RUX2; MTT and cell growth rate assay were used to detect the proliferation of periodontal ligament stem cells.
RESULTS AND CONCLUSION: Periodontal ligament stem cells were spindle-shaped and showed a higher
colony forming efficiency than periodontal ligament cells. The expressions of surface antigens of periodontal ligament stem cells-CD105, CD29, CD45, CD44 were respectively 99.8%, 99.7%, 1.26%, 98.8%, indicating periodontal ligament stem cells have the multilineage differentiation potential. Enamel matrix derivatives improve the collagen synthesis and mineralization nodule formation of periodontal ligament stem cells in a time-dose dependent manner. They also can improve the expression of osteogensis-related genes collagen type I, osteocalcin, RUX2 and proliferation of periodontal ligament stem cells.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Periodontal Ligament, Stem Cells, Dental Enamel Proteins, Cell Proliferation, Cell Differentiation

CLC Number:

R394.2

Wang Shuang, Feng Pei-xun, Chen Yue, Zhang Hai-juan, Li Sha, Bao Qing-hong, Guan Li-min . Effect of enamel matrix derivatives on the differentiation and proliferation of human periodontal ligament stem cells [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(23): 3716-3722.

Figures/Tables 4

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