Human telomerase reverse transcriptase gene-modified umbilical cord mesenchymal stem cell transplantation for acute kidney injury

doi:10.3969/j.issn.2095-4344.2015.23.014

Abstract

Abstract:

BACKGROUND: The human telomerase reverse transcriptase (hTERT) is one of preferred growth factors for regulating proliferation and directional differentiation, has multiple biological effects, and laids the foundation for genetically engineered immortalized stem cell lines.
OBJECTIVE: To investigate the effect of hTERT gene-modified umbilical cord mesenchymal stem cell transplantation on acute kidney injury induced by ischemia and reperfusion in rats.
METHODS: The human umbilical cord mesenchymal stem cells were cultured in vitro. Rat models of acute kidney injury induced by ischemia and reperfusion were established. Rat models were randomly divided into three groups. Rats in the control group were injected with 1 mL L-DMEM medium through caudal vein. Rats in the negative transfection group were injected with 1 mL umbilical cord mesenchymal stem cell suspension after empty virus transfection through caudal vein. Rats in the hTERT transfection group were injected with 1 mL umbilical cord mesenchymal stem cell suspension after PLXSN-hTERT transfection through caudal vein.
RESULTS AND CONCLUSION: At 3 and 28 days after transplantation, hematoxylin-eosin staining showed renal tubular damage score in the hTERT transfection group < negative transfection group < control group (P < 0.05). At 28 days after transplantation, the number of CM-Dil-positive cells in the hTERT transfection group > negative transfection group > control group (P < 0.05). At 1, 3, 14, and 28 days, serum creatinine and urea nitrogen levels
in the hTERT transfection group < negative transfection group < control group (P < 0.05). The results confirm that hTERT gene-modified umbilical cord mesenchymal stem cell transplantation has a significant repair effect on acute kidney injury in rats.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

Key words: Tissue Engineering, Stem Cells, Umbilical Cord Mesenchymal Stem Cells, Rats, Renal Injury

CLC Number:

R318

Wang Tian-sheng, Zhang Jian-jun. Human telomerase reverse transcriptase gene-modified umbilical cord mesenchymal stem cell transplantation for acute kidney injury [J]. Chinese Journal of Tissue Engineering Research, 2015, 19(23): 3686-3691.

Figures/Tables 4

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