Cx43 expression in the femur of rabbit models of steroid-induced vascular necrosis of the femoral head

doi:10.3969/j.issn.2095-4344.2015.18.005

Abstract

Abstract:

BACKGROUND: The mechanism of steroid-induced avascular necrosis of the femoral head is still unclear, Cx43 protein as the main gap junction in bone tissue, through transmitting information between osteoblasts, regulates bone cell growth and differentiation, compensatory bone increase or decrease. The relationship between Cx43 protein and steroid-induced avascular necrosis of the femoral head is still rarely reported.
OBJECTIVE: To explore the changes in Cx43 expression in rabbit models of steroid-induced vascular necrosis of the femoral head.
METHODS: Forty New Zealand rabbits were equally and randomly divided into model group and control group. Rabbits in the model group were used to establish models of steroid-induced avascular necrosis of the femoral head using endotoxin and hormone. Rabbits in the control group were injected with the same volume of physiological saline at the same time points.
RESULTS AND CONCLUSION: At 4 weeks after model establishment, hematoxylin-eosin staining results revealed that in the model group, the trabecula became thin and distributed disorderly in the femoral subchondral area. Empty lacuna increased significantly. Adipocytes increased. Hematopoietic cells in medullary cavity apparently diminished. In the control group, trabecula arranged orderly and empty lacuna could be seen. Bone marrow cells were abundant, but adipocytes were less. Immunohistochemical method demonstrated that Cx43 protein expression was observed in osteoblasts of the edge of trabecula, cytoplasm of osteoblasts of trabecula, and bone marrow stromal cells. Western blot assay results showed that alkaline phosphatase and Cx43 protein expression was lower in the model group than in the control group (P < 0.05). Results indicated that Cx43 protein expression decreased in the model rabbits, which may be the key link of the occurrence and development of steroid-induced avascular necrosis of the femoral head.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Tissue Engineering, Femur Head Necrosis, Hormones, Alkaline Phosphatase

CLC Number:

R318

Wei Lu, Luo Gao-bin, Li Wei, Lin Yi-cai, Ruan Zhong-jian, Zhou Zhi-guang, Bo Zhan-dong. Cx43 expression in the femur of rabbit models of steroid-induced vascular necrosis of the femoral head[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(18): 2814-2819.

Figures/Tables 2

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