Triggering receptor expressed on myeloid cells 2 in synovial tissue of rheumatoid arthritis rats

doi:10.3969/j.issn.2095-4344.2015.18.004

Abstract

Abstract:

BACKGROUND: Triggering receptor expressed on myeloid cells 2 (TREM-2) is highly expressed throughout the synovial tissue in active rheumatoid arthritis patients, but the role of TREM-2 in the pathogenesis of rheumatoid arthritis still remains unclear.
OBJECTIVE: To explore the TREM-2 expression in the synovial tissue of collagen type II-induced arthritis rats.
METHODS: The collagen-induced arthritis models were established in rats. The activity indicators and pathological changes of arthritis synovial were dynamically observed. The mRNA levels of TREM-2, tumor necrosis factor-α, interleukin-1β, and interleukin-10 were detected in synovial tissue of rats by RT-PCR. The protein expression and location of TREM-2 were measured with western blot assay and immunohistochemistry, respectively.
RESULTS AND CONCLUSION: At day 13 after immunization, the paws of model rats appeared red and swelling, the arthritis index scores were increased (P < 0.01). At day 19-25 after immunization, the inflammation reached the peak. Hematoxylin-eosin staining showed that, the synovium of collagen-induced arthritis rats were proliferated and were infiltrated by inflammatory cells, cartilage was destroyed. Compared with the control group, the expression of TREM-2 mRNA and protein, the mRNA levels of tumor necrosis factor-α and interleukin-1β in synovial tissue of the model rats were significantly increased (P < 0.05 or P < 0.01), while interleukin-10 mRNA expression was significantly decreased
(P < 0.05). Experimental findings indicate that, TREM-2 is a crucial inflammatory regulator and the increasing expression of TREM-2 plays an important role in the pathogenesis of collagen-induced arthritis.

中国组织工程研究杂志出版内容重点：肾移植；肝移植；移植；心脏移植；组织移植；皮肤移植；皮瓣移植；血管移植；器官移植；组织工程

全文链接：

Key words: Tissue Engineering, Arthritis, Rheumatoid, Arthritis, Experimental, Synovial Membrane, Cytokines

CLC Number:

R318

Ye Pei, Li Jian-hua, Xu Jin-huang, Huang Sheng-hui, Liu Gui-wang, Zhang Wei-qiong, Zheng Pei-zhong, Huang Jian-rong. Triggering receptor expressed on myeloid cells 2 in synovial tissue of rheumatoid arthritis rats[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(18): 2807-2813.

Figures/Tables 3

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