Transplantation of bone marrow stromal stem cells into the ischemic myocardium reduces myocardial apoptosis but cannot improve cardiac function

doi:10.3969/j.issn.2095-4344.2015.01.019

Abstract

Abstract:

BACKGROUND: Bone marrow stromal stem cells transplanted into infracted cardiac tissue can inhibit and reduce myocardial apoptosis, but whether this effect is correlated with improvement in cardiac function is still unclear.
OBJECTIVE: To study the early effect of bone marrow stromal stem cells transplanted into the ischemic myocardium on the cardiac function.
METHODS: Models of acute myocardial infarction were established by ligation of the left anterior descending branch, while no ligation was done in the sham group. In the transplantation group, rat bone marrow stromal stem cells (0.1 mL, 2×10⁶) were injected into five sites on the edge of infarcted myocardial tissues at 30 minutes after myocardial infarction. In the sham group and model group, the same volume of normal saline was injected into the myocardial tissues. Three days after cell transplantation, hemodynamic monitoring, echocardiography, TUNEL assay were employed to detect myocardial apoptosis.
RESULTS AND CONCLUSION: At 3 days after cell transplantation, myocardial apoptosis was more evident in the infarct and ischemic zones of the model group than the sham group; the number of apoptotic myocardial cells was significantly lower in the infarct and ischemic zones of the transplantation group than the model group. Compared with the sham group, the mean arterial blood pressure and left ventricular systolic pressure were significantly reduced, the left ventricular end diastolic pressure was increased, and the left ventricular ejection fraction and shortened fraction were significantly lowered in the model and transplantation groups (P < 0.05), but there was no difference between the model and transplantation groups (P > 0.05). These findings indicate that myocardial apoptosis can be reduced but the cardiac function cannot be improved in acute myocardial infarction rats at early stage after bone marrow stromal stem cells transplantation.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Bone Marrow, Stem Cells, Myocardial Infarction, Apoptosis

CLC Number:

R394.2

Jiang Shuai, Dong Shi-cai, Wei Dong-xing. Transplantation of bone marrow stromal stem cells into the ischemic myocardium reduces myocardial apoptosis but cannot improve cardiac function[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 108-113.

Figures/Tables 4

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