Ex-vivo expansion of autologous adipose-derived stem cells for the recovery of nasal mucosal function

doi:10.3969/j.issn.2095-4344.2015.01.013

Abstract

Abstract:

BACKGROUND: The ex-vivo expanded autologous adipose-derived stem cells have the capability of multipotential differentiation and have a broad application prospect in the field of tissue engineering and regenerative medicine.
OBJECTIVE: To observe the nasal mucosal structural repair and functional reconstruction using ex-vivo expanded autologous adipose-derived stem cells.
METHODS: Ten patients with mucosal damage due to the physical or chemical factors were enrolled, including six cases of mucosal scar and four cases of mucosal ulceration. Autologous adipose tissue was extracted for in vitro isolation, culture and expansion of adipose-derived stem cells. Before transplantation, quality safety testing was done. All the patients were injected adipose-derived stem cells (1×107/cm2) at an interval of 15 days, totally for three times. Nasal volume, minimum cross-sectional area, and mucociliary clearance function were determined at 30, 90, 150 days after the final injection. Three of 10 patients were selected to take a 0.1 cm×0.1 cm mucosal tissue sample at 30 days before and after transplantation for hematoxylin-eosin staining, Masson trichrome staining, and AB-PAS staining.
RESULTS AND CONCLUSION: Clinical symptoms were alleviated in all patients undergoing transplantation of adipose-derived stem cells. Compared with the baseline data, the nasal volume and minimum cross-sectional area were both decreased at 30, 90, 150 days after transplantation (P < 0.05), and the mucociliary clearance function was improved but not significantly (P > 0.05). Compared with the baseline data, the inflammation of the nasal mucosa was significantly reduced, collagen fibers arranged neatly, the deposition was decreased, and mucin secreted from goblet cells was increased in the selected three patients at 30 days after cell transplantation. These findings indicate that ex-vivo expanded autologous adipose-derived stem cells can be used to reconstruct the nasal mucosal structure and its function.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Stem Cells, Adipose Tissue, Nasal Mucosa, Mucociliary Clearance

CLC Number:

R394.2

Liu Yang, Jia De-jin, Yan Jun-ling, Li Liang, Chen Chong, Wang Cheng, Ding Hong, Tang Su-yang. Ex-vivo expansion of autologous adipose-derived stem cells for the recovery of nasal mucosal function[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 72-77.

Figures/Tables 4

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