Bone marrow mesenchymal stem cells combined with peptide hydrogel and chondrogenic factors for repair of articular cartilage defects in rabbits

doi:10.3969/j.issn.2095-4344.2015.01.006

Abstract

Abstract:

BACKGROUND: Peptide hydrogel has good plasticity, and it can fill the injured site very well; therefore, to use this material as a scaffold is a feasible exploration in bone and cartilage tissue engineering.
OBJECTIVE: To test the effect of bone marrow mesenchymal stem cells combined with injectable peptide hydrogel and chondrogenic factors for repair of articular cartilage defects in rabbits.
METHODS: Bone marrow mesenchymal stem cells from rabbits were isolated and cultured. A full-thickness bone-cartilage defect model, 5 mm in diameter and 3 mm in depth, was made on the left knee joint of rabbits, and the right knee joint of rabbits with no treatment was used as control after modeling. There were three experimental groups: self-assembling peptide hydrogel group, peptide hydrogel+chondrogenic factors group, and peptide hydrogel+chondrogenic factors+bone marrow mesenchymal stem cells group. Transforming growth factor β1, dexamethasone and insulin-like growth factor 1 were mixed as chondrogenic factors and added into self-assembling peptide hydrogel or bone marrow mesenchymal stem cells. Twelve weeks after treatment, animals were sacrificed for gross and histological observation, X-ray radiography, and histological evaluation using immunohistochemistry method.
RESULTS AND CONCLUSION: At 12 weeks after treatment, the self-assembling peptide hydrogel group showed excellent results in the cartilage repair, and better achievements in safranin-O staining, collagen II immunostaining, and histological scores than the other groups (P < 0.05); the peptide hydrogel+chondrogenic factors group had better repair effects similar to the self-assembling peptide hydrogel group, but the expression of proteoglycans was higher than that in the control group (P < 0.01); there were poorer repair effects and more osteophytes in the peptide hydrogel+chondrogenic factors+bone marrow mesenchymal stem cells group than the the peptide hydrogel+ chondrogenic factors group. Experimental findings indicate that the self-assembling peptide hydrogel can repair cartilage defects in situ and improve cartilage repair, which is expected to improve current repairing effects on cartilage defects.

中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程

全文链接：

Key words: Mesenchymal Stem Cells, Cytokines, Cartilage

CLC Number:

R394.2

Wang Yan, Li De-hua. Bone marrow mesenchymal stem cells combined with peptide hydrogel and chondrogenic factors for repair of articular cartilage defects in rabbits[J]. Chinese Journal of Tissue Engineering Research, 2015, 19(1): 30-36.

Figures/Tables 5

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